LOCUS       BBa_K245008             2261 bp    DNA     linear       25-Nov-2024
DEFINITION  BB-NIC-III-HisN
ACCESSION   BBa_K245008
VERSION     BBa_K245008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=AgeI
     misc_feature    1..82
                     /label=modified suffix
     misc_feature    7..12
                     /label=BspEI
     misc_feature    13..18
                     /label=SpeI
     misc_feature    19..21
                     /label=STOP
     stem_loop       22..69
                     /label=T7 terminator
     misc_feature    70..77
                     /label=NotI
     misc_feature    77..82
                     /label=PstI
     misc_feature    244..863
                     /label=pBR233 origin
     CDS             1018..1878
                     /label=amp resistance marker
     promoter        1920..1948
                     /label=amp promoter
     misc_feature    2119..2124
                     /label=EcoRI
     misc_feature    2119..2261
                     /label=modified prefix
     misc_feature    2125..2132
                     /label=NotI
     promoter        2133..2151
                     /label=T7 promoter
     misc_feature    2151..2178
                     /label=lacO regulatory site
     RBS             2208..2214
                     /label=rbs
     misc_feature    2220..2222
                     /label=START
     misc_feature    2226..2243
                     /label=His tag
     misc_feature    2244..2249
                     /label=XbaI
     misc_feature    2250..2255
                     /label=NgoMIV
     misc_feature    2256..2261
                     /label=XmaI
ORIGIN
        1 accggttccg gaactagtta actagcataa ccccttgggg cctctaaacg ggtgtagagg
       61 ggttttttgg cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
      121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      661 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
      721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      961 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1021 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1081 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1141 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1201 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1261 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1321 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1381 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1441 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1501 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1561 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1621 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1681 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1741 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1801 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1861 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1921 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1981 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     2041 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     2101 cgctaaggat gatttctgga attcgcggcc gctaatacga ctcactatag gggaattgtg
     2161 agcggataac aattccccgc tagaaataat tttgtttaac tttaagaagg aggtaaataa
     2221 tgtatcatca ccatcaccat cactctagag ccggccccgg g
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