##gff-version 3 ##sequence-region BBa_K245008 1 2261 BBa_K245008 . sequence_feature 77 82 . + 0 ID=annotation2027200;Name=PstI BBa_K245008 . start_codon 2220 2222 . + 0 ID=annotation2027210;Name=START BBa_K245008 . sequence_feature 7 12 . + 0 ID=annotation2027195;Name=BspEI BBa_K245008 . sequence_feature 70 77 . + 0 ID=annotation2027199;Name=NotI BBa_K245008 . sequence_feature 2244 2249 . + 0 ID=annotation2027212;Name=XbaI BBa_K245008 . stem_loop 22 69 . + 0 ID=annotation2027198;Name=T7 terminator BBa_K245008 . sequence_feature 2125 2132 . + 0 ID=annotation2027206;Name=NotI BBa_K245008 . sequence_feature 1 6 . + 0 ID=annotation2027193;Name=AgeI BBa_K245008 . promoter 1920 1948 . + 0 ID=annotation2027203;Name=amp promoter BBa_K245008 . sequence_feature 2256 2261 . + 0 ID=annotation2027214;Name=XmaI BBa_K245008 . sequence_feature 2119 2261 . + 0 ID=annotation2027205;Name=modified prefix BBa_K245008 . sequence_feature 13 18 . + 0 ID=annotation2027196;Name=SpeI BBa_K245008 . CDS 1018 1878 . + 0 ID=annotation2027202;Name=amp resistance marker BBa_K245008 . sequence_feature 2119 2124 . + 0 ID=annotation2027204;Name=EcoRI BBa_K245008 . sequence_feature 244 863 . + 0 ID=annotation2027201;Name=pBR233 origin BBa_K245008 . non_covalent_binding_site 2151 2178 . + 0 ID=annotation2027208;Name=lacO regulatory site BBa_K245008 . ribosome_entry_site 2208 2214 . + 0 ID=annotation2027209;Name=rbs BBa_K245008 . sequence_feature 2250 2255 . + 0 ID=annotation2027213;Name=NgoMIV BBa_K245008 . sequence_feature 1 82 . + 0 ID=annotation2027194;Name=modified suffix BBa_K245008 . tag 2226 2243 . + 0 ID=annotation2027211;Name=His tag BBa_K245008 . promoter 2133 2151 . + 0 ID=annotation2027207;Name=T7 promoter BBa_K245008 . stop_codon 19 21 . + 0 ID=annotation2027197;Name=STOP >BBa_K245008 accggttccggaactagttaactagcataaccccttggggcctctaaacgggtgtagaggggttttttggcggccgctg caggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaa tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagagg tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatct cagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagca gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttgg tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttcta cggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcaccta gatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgc ttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataa ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatt aattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcg tggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccat gttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatg gttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaacca agtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatag cagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcc agttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaa caggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaata ttattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg gttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaata ggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgct aatacgactcactataggggaattgtgagcggataacaattccccgctagaaataattttgtttaactttaagaaggag gtaaataatgtatcatcaccatcaccatcactctagagccggccccggg