BBa_K245008 1 BBa_K245008 BB-NIC-III-HisN 2009-09-25T11:00:00Z 2015-05-08T01:11:38Z The basis for BB-NIC-III-HisN is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_K245004. This plasmid backbone is the same as BBa_K245004 except that the restriction sites AgeI and XmaI are exchanged (which results in NgoMIV and XmaI restriction sites in prefix AgeI and BspEI in suffix). This detail enables extension of part cloned into vector with SG on C-terminal or/and PG on N-terminal. false false _337_ 0 4964 9 Not in stock false ccdB was multiplied from BB-NIC-II-HisN (BBa_K245004) by PCR using primers which contained restriction sites in different combinations than those found in BB-NIC-II-HisN next to ccdB. Fragment was then ligated in BB-NIC-II-HisN cut with NgoMIV and SpeI. false Nika Debeljak annotation2027200 1 PstI range2027200 1 77 82 annotation2027196 1 SpeI range2027196 1 13 18 annotation2027194 1 modified suffix range2027194 1 1 82 annotation2027207 1 T7 promoter range2027207 1 2133 2151 annotation2027204 1 EcoRI range2027204 1 2119 2124 annotation2027198 1 T7 terminator range2027198 1 22 69 annotation2027201 1 pBR233 origin range2027201 1 244 863 annotation2027205 1 modified prefix range2027205 1 2119 2261 annotation2027209 1 rbs range2027209 1 2208 2214 annotation2027206 1 NotI range2027206 1 2125 2132 annotation2027195 1 BspEI range2027195 1 7 12 annotation2027197 1 STOP range2027197 1 19 21 annotation2027211 1 His tag range2027211 1 2226 2243 annotation2027212 1 XbaI range2027212 1 2244 2249 annotation2027214 1 XmaI range2027214 1 2256 2261 annotation2027202 1 amp resistance marker range2027202 1 1018 1878 annotation2027199 1 NotI range2027199 1 70 77 annotation2027208 1 lacO regulatory site range2027208 1 2151 2178 annotation2027193 1 AgeI range2027193 1 1 6 annotation2027210 1 START range2027210 1 2220 2222 annotation2027213 1 NgoMIV range2027213 1 2250 2255 annotation2027203 1 amp promoter range2027203 1 1920 1948 BBa_K245008_sequence 1 accggttccggaactagttaactagcataaccccttggggcctctaaacgggtgtagaggggttttttggcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgctaatacgactcactataggggaattgtgagcggataacaattccccgctagaaataattttgtttaactttaagaaggaggtaaataatgtatcatcaccatcaccatcactctagagccggccccggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z