BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
BBa_K247003
1
BBa_K247003
ptet-rbs-mutant LuxR(Dull)
2009-10-18T11:00:00Z
2015-05-08T01:11:40Z
BBa_J13002 + BBa_K247001
This part use mutant LuxR(BBa_K247001).
false
false
_347_
0
5291
9
It's complicated
true
false
Kaoru Yamamoto, Yoshimi Iyama
component2052649
1
BBa_K247001
component2052642
1
BBa_R0040
component2052648
1
BBa_B0034
annotation2052642
1
BBa_R0040
range2052642
1
1
54
annotation2052648
1
BBa_B0034
range2052648
1
63
74
annotation2052649
1
BBa_K247001
range2052649
1
81
836
BBa_K247001
1
BBa_K247001
mutant LuxR(Dull)
2009-10-18T11:00:00Z
2015-05-08T01:11:40Z
A51G(synonymous), A77G(Asn->Ser), T303C(synonymous), A342C(Lys->Asn), A429G(synonymous), G629A(Ser->Asn), A655T(Thr->Ser)
This part is used in quorum sensing.
This LuxR can activate Lux promoter at high AHL conc.
false
false
_347_
0
5291
9
Not in stock
false
false
Kaoru Yamamoto
BBa_K247001_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaggcttgtagaagcaataatgatattagtcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgccgtaaataaaaaatctccaaatgtaattaaagaagcgaacacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaggacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcaatgagcgtactgtcactttccatttatccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K247003_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaggcttgtagaagcaataatgatattagtcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgccgtaaataaaaaatctccaaatgtaattaaagaagcgaacacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaggacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcaatgagcgtactgtcactttccatttatccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z