##gff-version 3
##sequence-region BBa_K248003 1 1159
BBa_K248003	.	promoter	1	312	.	+	0	ID=BBa_K248002;Name=BBa_K248002
BBa_K248003	.	promoter	1	312	.	+	0	ID=annotation2047306;Name=nirK;Parent=BBa_K248002
BBa_K248003	.	engineered_region	321	1159	.	+	0	ID=BBa_P0340;Name=BBa_P0340
BBa_K248003	.	ribosome_entry_site	321	331	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0340
BBa_K248003	.	engineered_region	321	331	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K248003	.	ribosome_entry_site	327	330	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K248003	.	ribosome_entry_site	321	331	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K248003	.	terminator	1119	1159	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0340
BBa_K248003	.	polyA_site	1146	1159	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K248003	.	stem_loop	1126	1145	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K248003	.	engineered_region	1119	1159	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K248003	.	stop_codon	1152	1152	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K248003	.	CDS	338	997	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0340
BBa_K248003	.	engineered_tag	998	1022	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K248003	.	CDS	341	957	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K248003	.	tag	958	991	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K248003	.	terminator	1031	1110	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0340
BBa_K248003	.	stem_loop	1042	1085	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K248003	.	engineered_region	1031	1110	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K248003
gttgcgcgaaataccgtaacaatccgcaatttccgtaatggtggacaattcctctcttttcaaaccgagataagtgagg
atgcgtaacgcgtaatcgctgtaattcgtcagtctcatgaagtattacccatttattgagtgggggttaacaagtatat
ccccacttttttatttctgcccgactggttgacaatctgaatcgaacgggtaaaatccatcaaaagatatattaataat
atatctttaagaggaggtggttaatgatcaccgaagaagttaaatatgactgtatttaaaggtctttcgtgcagatact
agagtcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgagg
tcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaa
aaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggg
gaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaag
tacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttt
ttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcat
caagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttg
atcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtga
aagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata