LOCUS       BBa_K248004              937 bp    DNA     linear       05-Nov-2024
DEFINITION  pPhoA-RBS-TetR
ACCESSION   BBa_K248004
VERSION     BBa_K248004.1  
FEATURES             Location/Qualifiers
     promoter        1..90
                     /label=phoA
     promoter        1..90
                     /label=BBa_K248000
     RBS             99..109
                     /label=RBS-4\Weaker
     RBS             105..108
                     /label=RBS
     misc_feature    99..109
                     /label=BBa_B0033
     RBS             99..109
                     /label=BBa_B0033
     CDS             119..735
                     /label=tetR
     misc_feature    736..769
                     /label=SsrA
     misc_feature    776..800
                     /label=Help:Barcodes
     CDS             116..775
                     /label=BBa_C0040
     stem_loop       820..863
                     /label=stem_loop
     misc_feature    809..888
                     /label=BBa_B0010
     terminator      809..888
                     /label=BBa_B0010
     stem_loop       904..923
                     /label=T7 TE
     misc_feature    930..930
                     /label=stop
     polyA_site      924..937
                     /label=polya
     misc_feature    897..937
                     /label=BBa_B0012
     terminator      897..937
                     /label=BBa_B0012
     misc_feature    99..937
                     /label=BBa_P0340
ORIGIN
        1 ttttcaacag ctgtcataaa gttgtcacgg ccgagactta tagtcgcttt gtttttattt
       61 tttaatgtat ttgtacatgg agaaaataaa tactagagtc acacaggact actagatgtc
      121 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
      181 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
      241 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
      301 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
      361 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
      421 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
      481 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
      541 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
      601 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
      661 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
      721 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg
      781 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      901 actggctcac cttcgggtgg gcctttctgc gtttata
//