##gff-version 3
##sequence-region BBa_K248004 1 937
BBa_K248004	.	promoter	1	90	.	+	0	ID=BBa_K248000;Name=BBa_K248000
BBa_K248004	.	promoter	1	90	.	+	0	ID=annotation2046024;Name=phoA;Parent=BBa_K248000
BBa_K248004	.	engineered_region	99	937	.	+	0	ID=BBa_P0340;Name=BBa_P0340
BBa_K248004	.	terminator	809	888	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0340
BBa_K248004	.	stem_loop	820	863	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K248004	.	engineered_region	809	888	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K248004	.	ribosome_entry_site	99	109	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0340
BBa_K248004	.	ribosome_entry_site	105	108	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K248004	.	ribosome_entry_site	99	109	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K248004	.	engineered_region	99	109	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K248004	.	CDS	116	775	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0340
BBa_K248004	.	engineered_tag	776	800	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K248004	.	tag	736	769	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K248004	.	CDS	119	735	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K248004	.	terminator	897	937	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0340
BBa_K248004	.	engineered_region	897	937	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K248004	.	stop_codon	930	930	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K248004	.	stem_loop	904	923	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K248004	.	polyA_site	924	937	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K248004
ttttcaacagctgtcataaagttgtcacggccgagacttatagtcgctttgtttttattttttaatgtatttgtacatg
gagaaaataaatactagagtcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattag
agctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacatt
gtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttt
tgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatc
gcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttttt
atgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattg
gaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaag
ctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaa
acaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata