##gff-version 3
##sequence-region BBa_K249001 1 1040
BBa_K249001	.	engineered_region	139	1040	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_K249001	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K249001	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K249001	.	CDS	157	816	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K249001	.	engineered_tag	817	841	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K249001	.	tag	777	810	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K249001	.	CDS	160	776	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K249001	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K249001	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K249001	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K249001	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K249001	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K249001	.	promoter	987	1040	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K249001	.	promoter	1006	1011	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K249001	.	non_covalent_binding_site	1012	1030	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K249001	.	engineered_region	987	1040	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K249001	.	non_covalent_binding_site	987	1005	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K249001	.	promoter	1029	1034	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K249001	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K249001	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K249001	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K249001	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
>BBa_K249001
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacc
cgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacg
ccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacg
taataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctaca
gaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatg
cactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggga
aacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcc
ttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatag
agatactgagcac