LOCUS       BBa_K249011              973 bp    DNA     linear       23-May-2024
DEFINITION  C-terminal CFP --> Tet promoter
ACCESSION   BBa_K249011
VERSION     BBa_K249011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..836
                     /label=BBa_K249007
     stem_loop       856..899
                     /label=stem_loop
     misc_feature    845..924
                     /label=BBa_B0010
     terminator      845..924
                     /label=BBa_B0010
     stem_loop       940..959
                     /label=T7 TE
     misc_feature    966..966
                     /label=stop
     polyA_site      960..973
                     /label=polya
     misc_feature    933..973
                     /label=BBa_B0012
     terminator      933..973
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagcac tagagccgcc gccgccgccg ccgccgccgc cgctaatact
      841 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      901 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      961 ttctgcgttt ata
//