LOCUS       BBa_K249012              979 bp    DNA     linear       18-May-2024
DEFINITION  N-terminal YFP --> Tet promoter
ACCESSION   BBa_K249012
VERSION     BBa_K249012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..842
                     /label=BBa_K249008
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgccgcc gccgccgccg ccgccgccgc cgcactagaa
      121 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      181 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      241 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      301 tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc
      361 tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      421 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      481 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      541 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      601 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      661 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      721 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      781 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
      841 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttata
//