LOCUS       BBa_K249015             1959 bp    DNA     linear       18-May-2024
DEFINITION  C-terminal CFP --> Tet inverter
ACCESSION   BBa_K249015
VERSION     BBa_K249015.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             160..776
                     /label=tetR
     misc_feature    777..810
                     /label=SsrA
     misc_feature    817..841
                     /label=Help:Barcodes
     CDS             157..816
                     /label=BBa_C0040
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     misc_feature    987..1005
                     /label=TetR 1
     misc_feature    987..1040
                     /label=BBa_R0040
     promoter        1006..1011
                     /label=-35
     misc_feature    1012..1030
                     /label=TetR 2
     promoter        1029..1034
                     /label=-10
     promoter        987..1040
                     /label=BBa_R0040
     misc_feature    139..1040
                     /label=BBa_Q04400
     misc_feature    1..1040
                     /label=BBa_K249001
     misc_feature    1053..1056
                     /label=conserved
     RBS             1049..1060
                     /label=BBa_B0034
     misc_feature    1067..1822
                     /label=BBa_K249007
     stem_loop       1842..1885
                     /label=stem_loop
     misc_feature    1831..1910
                     /label=BBa_B0010
     terminator      1831..1910
                     /label=BBa_B0010
     stem_loop       1926..1945
                     /label=T7 TE
     misc_feature    1952..1952
                     /label=stop
     polyA_site      1946..1959
                     /label=polya
     misc_feature    1919..1959
                     /label=BBa_B0012
     terminator      1919..1959
                     /label=BBa_B0012
     terminator      1831..1959
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa
      181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
      241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
      301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
      361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
      421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
      481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
      541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
      601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
      661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
      721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
      781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca
     1021 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgg tgagcaaggg
     1081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg
     1141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1201 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1261 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1501 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     1561 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1681 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcactaga gccgccgccg
     1801 ccgccgccgc cgccgccgct aatactagag ccaggcatca aataaaacga aaggctcagt
     1861 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1921 acactggctc accttcgggt gggcctttct gcgtttata
//