LOCUS       BBa_K249018             1965 bp    DNA     linear       22-Nov-2024
DEFINITION  N-terminal CFP --> Tet inverter
ACCESSION   BBa_K249018
VERSION     BBa_K249018.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             160..776
                     /label=tetR
     misc_feature    777..810
                     /label=SsrA
     misc_feature    817..841
                     /label=Help:Barcodes
     CDS             157..816
                     /label=BBa_C0040
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     misc_feature    987..1005
                     /label=TetR 1
     misc_feature    987..1040
                     /label=BBa_R0040
     promoter        1006..1011
                     /label=-35
     misc_feature    1012..1030
                     /label=TetR 2
     promoter        1029..1034
                     /label=-10
     promoter        987..1040
                     /label=BBa_R0040
     misc_feature    139..1040
                     /label=BBa_Q04400
     misc_feature    1..1040
                     /label=BBa_K249001
     misc_feature    1053..1056
                     /label=conserved
     RBS             1049..1060
                     /label=BBa_B0034
     misc_feature    1067..1828
                     /label=BBa_K249009
     stem_loop       1848..1891
                     /label=stem_loop
     misc_feature    1837..1916
                     /label=BBa_B0010
     terminator      1837..1916
                     /label=BBa_B0010
     stem_loop       1932..1951
                     /label=T7 TE
     misc_feature    1958..1958
                     /label=stop
     polyA_site      1952..1965
                     /label=polya
     misc_feature    1925..1965
                     /label=BBa_B0012
     terminator      1925..1965
                     /label=BBa_B0012
     terminator      1837..1965
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa
      181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
      241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
      301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
      361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
      421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
      481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
      541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
      601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
      661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
      721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
      781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca
     1021 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgc gccgccgccg
     1081 ccgccgccgc cgccgccgca ctagaatggt gagcaagggc gaggagctgt tcaccggggt
     1141 ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg
     1201 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1261 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
     1321 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1381 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1441 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1501 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta
     1561 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1621 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1681 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1741 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1801 cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg
     1861 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1921 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//