LOCUS BBa_K249024 3791 bp DNA linear 16-Dec-2024 DEFINITION Target N-terminal YFP and N-terminal CFP into Lumazine ACCESSION BBa_K249024 VERSION BBa_K249024.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..694 /label=BBa_K249002 stem_loop 714..757 /label=stem_loop misc_feature 703..782 /label=BBa_B0010 terminator 703..782 /label=BBa_B0010 stem_loop 798..817 /label=T7 TE misc_feature 824..824 /label=stop polyA_site 818..831 /label=polya misc_feature 791..831 /label=BBa_B0012 terminator 791..831 /label=BBa_B0012 misc_feature 1..831 /label=BBa_K249003 promoter 840..969 /label=BBa_I13453 misc_feature 982..985 /label=conserved RBS 978..989 /label=BBa_B0034 CDS 999..1615 /label=tetR misc_feature 1616..1649 /label=SsrA misc_feature 1656..1680 /label=Help:Barcodes CDS 996..1655 /label=BBa_C0040 stem_loop 1700..1743 /label=stem_loop misc_feature 1689..1768 /label=BBa_B0010 terminator 1689..1768 /label=BBa_B0010 stem_loop 1784..1803 /label=T7 TE misc_feature 1810..1810 /label=stop polyA_site 1804..1817 /label=polya misc_feature 1777..1817 /label=BBa_B0012 terminator 1777..1817 /label=BBa_B0012 misc_feature 1826..1844 /label=TetR 1 misc_feature 1826..1879 /label=BBa_R0040 promoter 1845..1850 /label=-35 misc_feature 1851..1869 /label=TetR 2 promoter 1868..1873 /label=-10 promoter 1826..1879 /label=BBa_R0040 misc_feature 978..1879 /label=BBa_Q04400 misc_feature 840..1879 /label=BBa_K249001 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 misc_feature 1906..2667 /label=BBa_K249008 stem_loop 2687..2730 /label=stem_loop misc_feature 2676..2755 /label=BBa_B0010 terminator 2676..2755 /label=BBa_B0010 stem_loop 2771..2790 /label=T7 TE misc_feature 2797..2797 /label=stop polyA_site 2791..2804 /label=polya misc_feature 2764..2804 /label=BBa_B0012 terminator 2764..2804 /label=BBa_B0012 terminator 2676..2804 /label=BBa_B0015 misc_feature 1..2804 /label=BBa_K249023 misc_feature 2813..2831 /label=TetR 1 misc_feature 2813..2866 /label=BBa_R0040 promoter 2832..2837 /label=-35 misc_feature 2838..2856 /label=TetR 2 promoter 2855..2860 /label=-10 promoter 2813..2866 /label=BBa_R0040 misc_feature 2879..2882 /label=conserved RBS 2875..2886 /label=BBa_B0034 misc_feature 2893..3654 /label=BBa_K249009 stem_loop 3674..3717 /label=stem_loop misc_feature 3663..3742 /label=BBa_B0010 terminator 3663..3742 /label=BBa_B0010 stem_loop 3758..3777 /label=T7 TE misc_feature 3784..3784 /label=stop polyA_site 3778..3791 /label=polya misc_feature 3751..3791 /label=BBa_B0012 terminator 3751..3791 /label=BBa_B0012 misc_feature 2813..3791 /label=BBa_K249013 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcagattta 241 tgaaggcaaa ctgaccgcgg aaggcctgcg ctttggcatt gtggcgagcc gctttaacca 301 tgcgctggtg gatcgcctgg tggaaggcgc gattgattgc attgtgcgcc atggtggtcg 361 cgaagaagat attaccctgg tgcgcgtgcc gggcagctgg gaaattccgg tggcggcggg 421 cgaactggcg cgcaaagaag atattgatgc ggtgattgcg attggcgtgc tgattgaagg 481 cgcggaaccg cattttgatt atattgcgag cgaagtgagc aaaggcctgg cgaacctgag 541 cctggaactg cgcaaaccga ttacctttgg cgtgattacc gcggatgaac tggaagaagc 601 gattgaacgc gcgggcacca aacatggcaa caaaggctgg gaagcggcgc tgagcgcgat 661 tgaaatggcg aacctgttta aaagcctgcg ctagtactag agccaggcat caaataaaac 721 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 781 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga 841 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 901 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat accgtttttt 961 tgggctagct actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag 1021 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 1081 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 1141 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 1201 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 1261 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 1321 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 1381 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 1441 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 1501 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 1561 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg 1621 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 1681 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1741 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1801 gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag 1861 tgatagagat actgagcact actagagaaa gaggagaaat actagatgcg ccgccgccgc 1921 cgccgccgcc gccgccgcac tagaatggtg agcaagggcg aggagctgtt caccggggtg 1981 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 2041 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 2101 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc 2161 gcccgctacc ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc 2221 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 2281 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 2341 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 2401 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 2461 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 2521 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc 2581 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 2641 ggcatggacg agctgtacaa gtaataatac tagagccagg catcaaataa aacgaaaggc 2701 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2761 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc 2821 agtgatagag attgacatcc ctatcagtga tagagatact gagcactact agagaaagag 2881 gagaaatact agatgcgccg ccgccgccgc cgccgccgcc gccgcactag aatggtgagc 2941 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgtg 3001 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 3061 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 3121 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 3181 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 3241 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 3301 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 3361 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag 3421 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 3481 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 3541 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3601 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag 3661 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3721 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3781 ctgcgtttat a //