##gff-version 3
##sequence-region BBa_K249024 1 3791
BBa_K249024	.	engineered_region	1	2804	.	+	0	ID=BBa_K249023;Name=BBa_K249023
BBa_K249024	.	engineered_region	1906	2667	.	+	0	ID=BBa_K249008;Name=BBa_K249008;Parent=BBa_K249023
BBa_K249024	.	ribosome_entry_site	1888	1899	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K249023
BBa_K249024	.	conserved_region	1892	1895	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K249024	.	engineered_region	1	831	.	+	0	ID=BBa_K249003;Name=BBa_K249003;Parent=BBa_K249023
BBa_K249024	.	terminator	703	782	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K249003
BBa_K249024	.	engineered_region	703	782	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K249024	.	stem_loop	714	757	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K249024	.	terminator	791	831	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K249003
BBa_K249024	.	engineered_region	791	831	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K249024	.	stop_codon	824	824	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K249024	.	polyA_site	818	831	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K249024	.	stem_loop	798	817	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K249024	.	CDS	230	694	.	+	0	ID=BBa_K249002;Name=BBa_K249002;Parent=BBa_K249003
BBa_K249024	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K249003
BBa_K249024	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K249024	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K249024	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K249024	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K249003
BBa_K249024	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K249024	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K249024	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K249024	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K249024	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K249024	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K249024	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K249024	.	engineered_region	840	1879	.	+	0	ID=BBa_K249001;Name=BBa_K249001;Parent=BBa_K249023
BBa_K249024	.	engineered_region	978	1879	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_K249001
BBa_K249024	.	CDS	996	1655	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K249024	.	tag	1616	1649	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K249024	.	engineered_tag	1656	1680	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K249024	.	CDS	999	1615	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K249024	.	ribosome_entry_site	978	989	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K249024	.	conserved_region	982	985	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K249024	.	promoter	1826	1879	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K249024	.	non_covalent_binding_site	1851	1869	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K249024	.	engineered_region	1826	1879	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K249024	.	non_covalent_binding_site	1826	1844	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K249024	.	promoter	1868	1873	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K249024	.	promoter	1845	1850	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K249024	.	terminator	1777	1817	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K249024	.	engineered_region	1777	1817	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K249024	.	stop_codon	1810	1810	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K249024	.	polyA_site	1804	1817	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K249024	.	stem_loop	1784	1803	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K249024	.	terminator	1689	1768	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K249024	.	engineered_region	1689	1768	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K249024	.	stem_loop	1700	1743	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K249024	.	promoter	840	969	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K249001
BBa_K249024	.	terminator	2676	2804	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K249023
BBa_K249024	.	terminator	2764	2804	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K249024	.	engineered_region	2764	2804	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K249024	.	stop_codon	2797	2797	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K249024	.	polyA_site	2791	2804	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K249024	.	stem_loop	2771	2790	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K249024	.	terminator	2676	2755	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K249024	.	engineered_region	2676	2755	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K249024	.	stem_loop	2687	2730	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K249024	.	engineered_region	2813	3791	.	+	0	ID=BBa_K249013;Name=BBa_K249013
BBa_K249024	.	terminator	3751	3791	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K249013
BBa_K249024	.	engineered_region	3751	3791	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K249024	.	stop_codon	3784	3784	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K249024	.	polyA_site	3778	3791	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K249024	.	stem_loop	3758	3777	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K249024	.	engineered_region	2893	3654	.	+	0	ID=BBa_K249009;Name=BBa_K249009;Parent=BBa_K249013
BBa_K249024	.	terminator	3663	3742	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K249013
BBa_K249024	.	engineered_region	3663	3742	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K249024	.	stem_loop	3674	3717	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K249024	.	promoter	2813	2866	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K249013
BBa_K249024	.	non_covalent_binding_site	2838	2856	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K249024	.	engineered_region	2813	2866	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K249024	.	non_covalent_binding_site	2813	2831	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K249024	.	promoter	2855	2860	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K249024	.	promoter	2832	2837	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K249024	.	ribosome_entry_site	2875	2886	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K249013
BBa_K249024	.	conserved_region	2879	2882	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K249024
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgcagat
ttatgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgc
ctggtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggca
gctgggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgat
tgaaggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgc
aaaccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaaca
aaggctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctagtactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacacttt
gctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctcc
ataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaa
cagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagag
cagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcacc
atactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgcttt
actaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaa
ttagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttag
gttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccatt
attacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgc
ggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacact
gatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggag
aaatactagatgcgccgccgccgccgccgccgccgccgccgcactagaatggtgagcaagggcgaggagctgttcaccg
gggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcga
tgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacc
accttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgc
ccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcga
gggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctg
gagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatcc
gccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgct
gctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctg
ctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctat
cagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgccgccgccgccgccgccgccgccgcc
gcactagaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaa
cggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcacc
accggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccg
accacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaagga
cgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatc
gacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccg
acaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgacca
ctaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctg
agcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatgg
acgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata