LOCUS BBa_K249025 3779 bp DNA linear 15-Dec-2024 DEFINITION Target C-terminal YFP and C-terminal CFP into Lumazine ACCESSION BBa_K249025 VERSION BBa_K249025.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..694 /label=BBa_K249002 stem_loop 714..757 /label=stem_loop misc_feature 703..782 /label=BBa_B0010 terminator 703..782 /label=BBa_B0010 stem_loop 798..817 /label=T7 TE misc_feature 824..824 /label=stop polyA_site 818..831 /label=polya misc_feature 791..831 /label=BBa_B0012 terminator 791..831 /label=BBa_B0012 misc_feature 1..831 /label=BBa_K249003 promoter 840..969 /label=BBa_I13453 misc_feature 982..985 /label=conserved RBS 978..989 /label=BBa_B0034 CDS 999..1615 /label=tetR misc_feature 1616..1649 /label=SsrA misc_feature 1656..1680 /label=Help:Barcodes CDS 996..1655 /label=BBa_C0040 stem_loop 1700..1743 /label=stem_loop misc_feature 1689..1768 /label=BBa_B0010 terminator 1689..1768 /label=BBa_B0010 stem_loop 1784..1803 /label=T7 TE misc_feature 1810..1810 /label=stop polyA_site 1804..1817 /label=polya misc_feature 1777..1817 /label=BBa_B0012 terminator 1777..1817 /label=BBa_B0012 misc_feature 1826..1844 /label=TetR 1 misc_feature 1826..1879 /label=BBa_R0040 promoter 1845..1850 /label=-35 misc_feature 1851..1869 /label=TetR 2 promoter 1868..1873 /label=-10 promoter 1826..1879 /label=BBa_R0040 misc_feature 978..1879 /label=BBa_Q04400 misc_feature 840..1879 /label=BBa_K249001 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 misc_feature 1906..2661 /label=BBa_K249006 stem_loop 2681..2724 /label=stem_loop misc_feature 2670..2749 /label=BBa_B0010 terminator 2670..2749 /label=BBa_B0010 stem_loop 2765..2784 /label=T7 TE misc_feature 2791..2791 /label=stop polyA_site 2785..2798 /label=polya misc_feature 2758..2798 /label=BBa_B0012 terminator 2758..2798 /label=BBa_B0012 terminator 2670..2798 /label=BBa_B0015 misc_feature 1..2798 /label=BBa_K249021 misc_feature 2807..2825 /label=TetR 1 misc_feature 2807..2860 /label=BBa_R0040 promoter 2826..2831 /label=-35 misc_feature 2832..2850 /label=TetR 2 promoter 2849..2854 /label=-10 promoter 2807..2860 /label=BBa_R0040 misc_feature 2873..2876 /label=conserved RBS 2869..2880 /label=BBa_B0034 misc_feature 2887..3642 /label=BBa_K249007 stem_loop 3662..3705 /label=stem_loop misc_feature 3651..3730 /label=BBa_B0010 terminator 3651..3730 /label=BBa_B0010 stem_loop 3746..3765 /label=T7 TE misc_feature 3772..3772 /label=stop polyA_site 3766..3779 /label=polya misc_feature 3739..3779 /label=BBa_B0012 terminator 3739..3779 /label=BBa_B0012 misc_feature 2807..3779 /label=BBa_K249011 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcagattta 241 tgaaggcaaa ctgaccgcgg aaggcctgcg ctttggcatt gtggcgagcc gctttaacca 301 tgcgctggtg gatcgcctgg tggaaggcgc gattgattgc attgtgcgcc atggtggtcg 361 cgaagaagat attaccctgg tgcgcgtgcc gggcagctgg gaaattccgg tggcggcggg 421 cgaactggcg cgcaaagaag atattgatgc ggtgattgcg attggcgtgc tgattgaagg 481 cgcggaaccg cattttgatt atattgcgag cgaagtgagc aaaggcctgg cgaacctgag 541 cctggaactg cgcaaaccga ttacctttgg cgtgattacc gcggatgaac tggaagaagc 601 gattgaacgc gcgggcacca aacatggcaa caaaggctgg gaagcggcgc tgagcgcgat 661 tgaaatggcg aacctgttta aaagcctgcg ctagtactag agccaggcat caaataaaac 721 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 781 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga 841 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 901 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat accgtttttt 961 tgggctagct actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag 1021 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 1081 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 1141 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 1201 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 1261 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 1321 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 1381 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 1441 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 1501 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 1561 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg 1621 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 1681 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1741 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1801 gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag 1861 tgatagagat actgagcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 1981 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 2041 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc 2101 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc 2161 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 2221 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2281 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 2341 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 2401 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 2461 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag 2521 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 2581 accgccgccg ggatcactct cggcatggac gagctgtaca agcactagag ccgccgccgc 2641 cgccgccgcc gccgccgcta atactagagc caggcatcaa ataaaacgaa aggctcagtc 2701 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2761 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat 2821 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa 2881 tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2941 ctggacggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 3001 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 3061 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac 3121 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 3181 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 3241 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 3301 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag 3361 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 3421 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 3481 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 3541 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 3601 aagcactaga gccgccgccg ccgccgccgc cgccgccgct aatactagag ccaggcatca 3661 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3721 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //