BBa_K249028 1 BBa_K249028 Theophylline Induced Chemotaxis 2009-08-25T11:00:00Z 2015-05-08T01:11:40Z existing BioBrick parts + synthesized DNA Chemotaxis by cheZ is controlled translationally by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing the production of cheZ, a key regulatory element in the motility pathway false false _342_ 0 3171 9 It's complicated false Standard false Roxanne Shank component2061073 1 BBa_K147000 component2061071 1 BBa_J23119 component2061074 1 BBa_B0010 component2061072 1 BBa_K249026 component2061076 1 BBa_B0012 annotation2061072 1 BBa_K249026 range2061072 1 44 102 annotation2061071 1 BBa_J23119 range2061071 1 1 35 annotation2061074 1 BBa_B0010 range2061074 1 954 1033 annotation2061076 1 BBa_B0012 range2061076 1 1042 1082 annotation2061073 1 BBa_K147000 range2061073 1 111 945 BBa_K249026 1 BBa_K249026 Theophylline Riboswitch (Improved) 2009-08-25T11:00:00Z 2015-05-08T01:11:40Z Synthesized DNA This is an improvement on the Theophylline Riboswitch submitted by the University of Lethbridge 2007 iGEM team. The theophylline Riboswithc binds to Theophylline in a specific manner to induce transcription of an mRNA. false false _342_ 0 3171 9 It's complicated false Standard false Roxanne Shank BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K147000 1 BBa_K147000 cheZ 2008-10-27T12:00:00Z 2015-05-08T01:10:38Z Amplified from pTopp plasmid. The cheZ motility protein is a protein kinase which dephosphorylates cheY. cheY regulates the clockwise and counterclockwise rotation of the motor flagella. If cheY is phosphorylated the motor flagella are not in sync and the bacterium tumbles. If cheY is not phosphorylated the motor flagella are in sync and the bacterium runs (often towards a chemoattractant) false false _242_ 0 3171 9 It's complicated false Nothing of note false Roxanne Shank BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K249028_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatgtactagagcgccaacatcctgatcggtgctgacacgcgtaacaatgacccccgcggcggtagaaatcaccagagccgggatttgtgccaccagaccgtcaccaatggtcaagagcgtataactttccgccgcgtgtcccatgctcatgccatgttgcagcacgccgaccagcaacccgccgacaacgttaatgaccatgatgaggatcccggcgatggcatcgccgcgaacaaacttacttgccccgtccattgagccgtaaaaatcggcttcctgagtgacttcggagcggcgttttttcgcctcatcttcaccaatcaatccggcgttaaggtcggcgtcaatcgccatctgcttacccggcataccatcgagaacaaagcgcgcacccacttctgcgatacgcccggcacctttggtaatgaccataaagttgatgatcacgagaatgacaaacaccacgataccgatagcgaaattgccaccaacgaggaagtgaccgaacgcttcaaccaccttccctgccgccgctgcgccggtatgcccttccattaaaatgatacgggttgaagccacgttaagtgccagacgcaacagcgtggtaaacaacaaaatggtcggaaacgcagcaaactcaagcgtgcgctgggtaaacatcgccaccagcaacaccatgatcgacaaggcaatattgaaggtaaacaacaggtcgagtatgaatgcgggcagtggcagcaccatcatcgacaagatcaacaggatcaaaatcggtccggcaaggatctgccattgtgtcgatttcaggtttgtgggcaggcgcagcatcgcggccagattactcattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K249026_sequence 1 ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K147000_sequence 1 cgccaacatcctgatcggtgctgacacgcgtaacaatgacccccgcggcggtagaaatcaccagagccgggatttgtgccaccagaccgtcaccaatggtcaagagcgtataactttccgccgcgtgtcccatgctcatgccatgttgcagcacgccgaccagcaacccgccgacaacgttaatgaccatgatgaggatcccggcgatggcatcgccgcgaacaaacttacttgccccgtccattgagccgtaaaaatcggcttcctgagtgacttcggagcggcgttttttcgcctcatcttcaccaatcaatccggcgttaaggtcggcgtcaatcgccatctgcttacccggcataccatcgagaacaaagcgcgcacccacttctgcgatacgcccggcacctttggtaatgaccataaagttgatgatcacgagaatgacaaacaccacgataccgatagcgaaattgccaccaacgaggaagtgaccgaacgcttcaaccaccttccctgccgccgctgcgccggtatgcccttccattaaaatgatacgggttgaagccacgttaagtgccagacgcaacagcgtggtaaacaacaaaatggtcggaaacgcagcaaactcaagcgtgcgctgggtaaacatcgccaccagcaacaccatgatcgacaaggcaatattgaaggtaaacaacaggtcgagtatgaatgcgggcagtggcagcaccatcatcgacaagatcaacaggatcaaaatcggtccggcaaggatctgccattgtgtcgatttcaggtttgtgggcaggcgcagcatcgcggccagattactcat BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z