##gff-version 3
##sequence-region BBa_K255001 1 3038
BBa_K255001	.	promoter	2099	2152	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K255001	.	promoter	2141	2146	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K255001	.	non_covalent_binding_site	2124	2142	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K255001	.	promoter	2118	2123	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K255001	.	engineered_region	2099	2152	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K255001	.	non_covalent_binding_site	2099	2117	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K255001	.	terminator	1962	2041	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K255001	.	stem_loop	1973	2016	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K255001	.	engineered_region	1962	2041	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K255001	.	ribosome_entry_site	2161	2172	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K255001	.	conserved_region	2165	2168	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K255001	.	terminator	2910	2989	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K255001	.	stem_loop	2921	2964	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K255001	.	engineered_region	2910	2989	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K255001	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K255001	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K255001	.	CDS	82	1953	.	+	0	ID=BBa_K255000;Name=BBa_K255000
BBa_K255001	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K255001	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K255001	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K255001	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K255001	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K255001	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K255001	.	CDS	2179	2901	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_K255001	.	terminator	2050	2090	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K255001	.	polyA_site	2077	2090	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K255001	.	stem_loop	2057	2076	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K255001	.	stop_codon	2083	2083	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K255001	.	engineered_region	2050	2090	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K255001	.	terminator	2998	3038	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K255001	.	polyA_site	3025	3038	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K255001	.	stem_loop	3005	3024	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K255001	.	stop_codon	3031	3031	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K255001	.	engineered_region	2998	3038	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K255001
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca
ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg
gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa
ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga
agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag
gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca
gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct
gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt
cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca
tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg
cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc
aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca
atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt
ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagctcgagggagaaggacaaggacaa
ggacaaggaccaggacgaggatacgcataccgaagcggttccggaatggtgagcaagggcgaggagctgttcaccgggg
tggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgc
cacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacc
ctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccg
aaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgaggg
cgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggag
tacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgcc
acaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgct
gcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctg
gagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccgggg
tggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgc
cacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacc
ttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccg
aaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgaggg
cgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggag
tacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgcc
acaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgct
gcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctg
gagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata