LOCUS BBa_K255003 3038 bp DNA linear 18-Nov-2024 DEFINITION pLac.lacI-cfp(fusion).terminator.pTet.yfp.terminator ACCESSION BBa_K255003 VERSION BBa_K255003.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..1953 /label=BBa_K255000 stem_loop 1973..2016 /label=stem_loop misc_feature 1962..2041 /label=BBa_B0010 terminator 1962..2041 /label=BBa_B0010 stem_loop 2057..2076 /label=T7 TE misc_feature 2083..2083 /label=stop polyA_site 2077..2090 /label=polya misc_feature 2050..2090 /label=BBa_B0012 terminator 2050..2090 /label=BBa_B0012 misc_feature 2099..2117 /label=TetR 1 misc_feature 2099..2152 /label=BBa_R0040 promoter 2118..2123 /label=-35 misc_feature 2124..2142 /label=TetR 2 promoter 2141..2146 /label=-10 promoter 2099..2152 /label=BBa_R0040 misc_feature 2165..2168 /label=conserved RBS 2161..2172 /label=BBa_B0034 CDS 2179..2901 /label=BBa_E0030 stem_loop 2921..2964 /label=stem_loop misc_feature 2910..2989 /label=BBa_B0010 terminator 2910..2989 /label=BBa_B0010 stem_loop 3005..3024 /label=T7 TE misc_feature 3031..3031 /label=stop polyA_site 3025..3038 /label=polya misc_feature 2998..3038 /label=BBa_B0012 terminator 2998..3038 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc 121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1141 tcccgactgg aaagcgggca gctcgaggga gaaggacaag gacaaggaca aggaccagga 1201 cgaggatacg cataccgaag cggttccgga atggtgagca agggcgagga gctgttcacc 1261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg 1321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag 1441 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 1501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 1561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 1621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac 1681 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac 1741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 1801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 1861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 1921 actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg 1981 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 2041 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc 2101 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag 2161 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 2221 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 2281 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 2341 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc 2401 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 2461 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 2521 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 2581 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 2641 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 2701 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 2761 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 2821 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 2881 gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 2941 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3001 cactggctca ccttcgggtg ggcctttctg cgtttata //