LOCUS       BBa_K256001             1069 bp    DNA     linear       16-Dec-2024
DEFINITION  pNO:GFP
ACCESSION   BBa_K256001
VERSION     BBa_K256001.1  
FEATURES             Location/Qualifiers
     promoter        1..186
                     /label=pNO
     promoter        1..186
                     /label=BBa_K256000
     misc_feature    199..202
                     /label=conserved
     RBS             195..206
                     /label=BBa_B0034
     CDS             213..932
                     /label=GFP protein
     CDS             213..932
                     /label=BBa_E0040
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
ORIGIN
        1 agcaacctca atttattcag cgtgttctaa aaagatgtct tgctaattgc agattgcgtg
       61 ccagtttttt atctgattgt ttttactcta taaataaaat tatgcccatt aatgagtagt
      121 caaaatgact atcaatcaaa tggtcgatat gacaatatct atagtcaaat tgacagtgag
      181 gcaaagtact agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg
      241 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      301 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      361 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
      421 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
      481 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
      541 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
      601 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
      661 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
      721 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
      781 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
      841 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
      901 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//