BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K256004 1 NorR NorR 2009-10-15T11:00:00Z 2015-05-08T01:11:40Z -- DNA-binding transcriptional activator, B2709, NorR, YgaA, transcriptional regulator of the anaerobic response to reactive nitrogen species false false _359_ 0 4864 9 Not in stock true -- false iGEM09_NTU-Singapore annotation2048002 1 misc range2048002 1 1 1515 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K256006 1 BBa_K256006 B0034:NorR:B0015 2009-10-15T11:00:00Z 2015-05-08T01:11:40Z -- Intermediate of NorR generator false false _359_ 0 4864 9 It's complicated false -- false iGEM09_NTU-Singapore component2048160 1 BBa_K256004 component2048161 1 BBa_B0010 component2048159 1 BBa_B0034 component2048163 1 BBa_B0012 annotation2048161 1 BBa_B0010 range2048161 1 1542 1621 annotation2048159 1 BBa_B0034 range2048159 1 1 12 annotation2048160 1 BBa_K256004 range2048160 1 19 1533 annotation2048163 1 BBa_B0012 range2048163 1 1630 1670 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K256004_sequence 1 atgagtttttccgttgatgtgctggcgaatatcgccatcgaattgcagcgtgggattggtcaccaggatcgttttcagcgcctgatcaccacgctacgtcaggtgctggagtgcgatgcgtctgcgttgctacgttacgattcgcggcagtttattccgcttgccatcgacggtctggcaaaggatgtactcggtagacgctttgcgctggaagggcatccacggctggaagcgattgcccgcgccggggatgtggtgcgctttcccgcagacagcgaattgcccgatccctatgacggtttgattcctgggcaggagagtctgaaggttcacgcctgcgttggtctgccattgtttgccgggcaaaacctgatcggcgcactgacgctcgacgggatgcagcccgatcagttcgatgttttcagcgacgaagagctacggctgattgctgcgctggcggcgggagcgttaagcaatgcgttgctgattgaacaactggaaagccagaatatgctgccaggcgatgccacgccgtttgaagcggtgaaacagacgcagatgattggcttgtcccctggcatgacgcaactgaaaaaagagattgagattgtggcggcgtccgatctcaacgtcctgatcagcggtgagactggaaccggtaaggagctggtggcgaaagcgattcatgaagcctcgccacgggcggtgaatccgctggtctatctcaactgtgctgcactgccggaaagtgtggcggaaagtgagttgttcgggcatgtgaaaggagcgtttactggcgctatcagtaatcgcagcgggaagtttgaaatggcggataacggcacgctgtttctggatgagatcggcgagttgtcgttggcattgcaggccaagctgctgagggtgttgcagtatggcgatattcagcgcgttggcgatgaccgttgtttgcgggtcgatgtgcgcgtgctggcggcgactaaccgcgatttacgcgaagaggtgctggcagggcgattccgcgccgatttgtttcatcgcctgagcgtgtttccactttcggtgccgccgctgcgtgagcggggcgatgatgtcattctgctggcggggtatttctgcgagcagtgtcgtttgcggcaggggctctcccgcgtggtattaagtgccggagcgcgaaatttactgcaacactacagttttccgggaaacgtgcgcgaactggaacatgctattcatcgggcggtagttctggcgagagccacccgcagcggcgatgaagtgattcttgaggcgcaacattttgcttttcctgaggtgacgttgccgacgccagaagtggcggcggtgcccgttgttaagcaaaacctgcgtgaagcgacagaagcgttccagcgtgaaactattcgtcaggcactggcacaaaatcatcacaactgggctgcctgcgcgcggatgctggaaaccgacgtcgccaacctgcatcggctggcgaaacgtctgggattgaaggattaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K256006_sequence 1 aaagaggagaaatactagatgagtttttccgttgatgtgctggcgaatatcgccatcgaattgcagcgtgggattggtcaccaggatcgttttcagcgcctgatcaccacgctacgtcaggtgctggagtgcgatgcgtctgcgttgctacgttacgattcgcggcagtttattccgcttgccatcgacggtctggcaaaggatgtactcggtagacgctttgcgctggaagggcatccacggctggaagcgattgcccgcgccggggatgtggtgcgctttcccgcagacagcgaattgcccgatccctatgacggtttgattcctgggcaggagagtctgaaggttcacgcctgcgttggtctgccattgtttgccgggcaaaacctgatcggcgcactgacgctcgacgggatgcagcccgatcagttcgatgttttcagcgacgaagagctacggctgattgctgcgctggcggcgggagcgttaagcaatgcgttgctgattgaacaactggaaagccagaatatgctgccaggcgatgccacgccgtttgaagcggtgaaacagacgcagatgattggcttgtcccctggcatgacgcaactgaaaaaagagattgagattgtggcggcgtccgatctcaacgtcctgatcagcggtgagactggaaccggtaaggagctggtggcgaaagcgattcatgaagcctcgccacgggcggtgaatccgctggtctatctcaactgtgctgcactgccggaaagtgtggcggaaagtgagttgttcgggcatgtgaaaggagcgtttactggcgctatcagtaatcgcagcgggaagtttgaaatggcggataacggcacgctgtttctggatgagatcggcgagttgtcgttggcattgcaggccaagctgctgagggtgttgcagtatggcgatattcagcgcgttggcgatgaccgttgtttgcgggtcgatgtgcgcgtgctggcggcgactaaccgcgatttacgcgaagaggtgctggcagggcgattccgcgccgatttgtttcatcgcctgagcgtgtttccactttcggtgccgccgctgcgtgagcggggcgatgatgtcattctgctggcggggtatttctgcgagcagtgtcgtttgcggcaggggctctcccgcgtggtattaagtgccggagcgcgaaatttactgcaacactacagttttccgggaaacgtgcgcgaactggaacatgctattcatcgggcggtagttctggcgagagccacccgcagcggcgatgaagtgattcttgaggcgcaacattttgcttttcctgaggtgacgttgccgacgccagaagtggcggcggtgcccgttgttaagcaaaacctgcgtgaagcgacagaagcgttccagcgtgaaactattcgtcaggcactggcacaaaatcatcacaactgggctgcctgcgcgcggatgctggaaaccgacgtcgccaacctgcatcggctggcgaaacgtctgggattgaaggattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z