BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K256007 1 BBa_K256007 J23119:NorR 2009-10-15T11:00:00Z 2015-05-08T01:11:41Z -- Generator of NorR false false _359_ 0 4864 9 It's complicated true -- false iGEM09_NTU-Singapore component2048169 1 BBa_B0034 component2048167 1 BBa_J23119 component2048170 1 BBa_K256004 component2048171 1 BBa_B0010 component2048173 1 BBa_B0012 annotation2048170 1 BBa_K256004 range2048170 1 62 1576 annotation2048167 1 BBa_J23119 range2048167 1 1 35 annotation2048169 1 BBa_B0034 range2048169 1 44 55 annotation2048171 1 BBa_B0010 range2048171 1 1585 1664 annotation2048173 1 BBa_B0012 range2048173 1 1673 1713 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K256004 1 NorR NorR 2009-10-15T11:00:00Z 2015-05-08T01:11:40Z -- DNA-binding transcriptional activator, B2709, NorR, YgaA, transcriptional regulator of the anaerobic response to reactive nitrogen species false false _359_ 0 4864 9 Not in stock true -- false iGEM09_NTU-Singapore annotation2048002 1 misc range2048002 1 1 1515 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K256004_sequence 1 atgagtttttccgttgatgtgctggcgaatatcgccatcgaattgcagcgtgggattggtcaccaggatcgttttcagcgcctgatcaccacgctacgtcaggtgctggagtgcgatgcgtctgcgttgctacgttacgattcgcggcagtttattccgcttgccatcgacggtctggcaaaggatgtactcggtagacgctttgcgctggaagggcatccacggctggaagcgattgcccgcgccggggatgtggtgcgctttcccgcagacagcgaattgcccgatccctatgacggtttgattcctgggcaggagagtctgaaggttcacgcctgcgttggtctgccattgtttgccgggcaaaacctgatcggcgcactgacgctcgacgggatgcagcccgatcagttcgatgttttcagcgacgaagagctacggctgattgctgcgctggcggcgggagcgttaagcaatgcgttgctgattgaacaactggaaagccagaatatgctgccaggcgatgccacgccgtttgaagcggtgaaacagacgcagatgattggcttgtcccctggcatgacgcaactgaaaaaagagattgagattgtggcggcgtccgatctcaacgtcctgatcagcggtgagactggaaccggtaaggagctggtggcgaaagcgattcatgaagcctcgccacgggcggtgaatccgctggtctatctcaactgtgctgcactgccggaaagtgtggcggaaagtgagttgttcgggcatgtgaaaggagcgtttactggcgctatcagtaatcgcagcgggaagtttgaaatggcggataacggcacgctgtttctggatgagatcggcgagttgtcgttggcattgcaggccaagctgctgagggtgttgcagtatggcgatattcagcgcgttggcgatgaccgttgtttgcgggtcgatgtgcgcgtgctggcggcgactaaccgcgatttacgcgaagaggtgctggcagggcgattccgcgccgatttgtttcatcgcctgagcgtgtttccactttcggtgccgccgctgcgtgagcggggcgatgatgtcattctgctggcggggtatttctgcgagcagtgtcgtttgcggcaggggctctcccgcgtggtattaagtgccggagcgcgaaatttactgcaacactacagttttccgggaaacgtgcgcgaactggaacatgctattcatcgggcggtagttctggcgagagccacccgcagcggcgatgaagtgattcttgaggcgcaacattttgcttttcctgaggtgacgttgccgacgccagaagtggcggcggtgcccgttgttaagcaaaacctgcgtgaagcgacagaagcgttccagcgtgaaactattcgtcaggcactggcacaaaatcatcacaactgggctgcctgcgcgcggatgctggaaaccgacgtcgccaacctgcatcggctggcgaaacgtctgggattgaaggattaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K256007_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgagtttttccgttgatgtgctggcgaatatcgccatcgaattgcagcgtgggattggtcaccaggatcgttttcagcgcctgatcaccacgctacgtcaggtgctggagtgcgatgcgtctgcgttgctacgttacgattcgcggcagtttattccgcttgccatcgacggtctggcaaaggatgtactcggtagacgctttgcgctggaagggcatccacggctggaagcgattgcccgcgccggggatgtggtgcgctttcccgcagacagcgaattgcccgatccctatgacggtttgattcctgggcaggagagtctgaaggttcacgcctgcgttggtctgccattgtttgccgggcaaaacctgatcggcgcactgacgctcgacgggatgcagcccgatcagttcgatgttttcagcgacgaagagctacggctgattgctgcgctggcggcgggagcgttaagcaatgcgttgctgattgaacaactggaaagccagaatatgctgccaggcgatgccacgccgtttgaagcggtgaaacagacgcagatgattggcttgtcccctggcatgacgcaactgaaaaaagagattgagattgtggcggcgtccgatctcaacgtcctgatcagcggtgagactggaaccggtaaggagctggtggcgaaagcgattcatgaagcctcgccacgggcggtgaatccgctggtctatctcaactgtgctgcactgccggaaagtgtggcggaaagtgagttgttcgggcatgtgaaaggagcgtttactggcgctatcagtaatcgcagcgggaagtttgaaatggcggataacggcacgctgtttctggatgagatcggcgagttgtcgttggcattgcaggccaagctgctgagggtgttgcagtatggcgatattcagcgcgttggcgatgaccgttgtttgcgggtcgatgtgcgcgtgctggcggcgactaaccgcgatttacgcgaagaggtgctggcagggcgattccgcgccgatttgtttcatcgcctgagcgtgtttccactttcggtgccgccgctgcgtgagcggggcgatgatgtcattctgctggcggggtatttctgcgagcagtgtcgtttgcggcaggggctctcccgcgtggtattaagtgccggagcgcgaaatttactgcaacactacagttttccgggaaacgtgcgcgaactggaacatgctattcatcgggcggtagttctggcgagagccacccgcagcggcgatgaagtgattcttgaggcgcaacattttgcttttcctgaggtgacgttgccgacgccagaagtggcggcggtgcccgttgttaagcaaaacctgcgtgaagcgacagaagcgttccagcgtgaaactattcgtcaggcactggcacaaaatcatcacaactgggctgcctgcgcgcggatgctggaaaccgacgtcgccaacctgcatcggctggcgaaacgtctgggattgaaggattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z