LOCUS       BBa_K256014             1652 bp    DNA     linear       05-Nov-2024
DEFINITION  B0034: HO1: B0034:GFP :B0015
ACCESSION   BBa_K256014
VERSION     BBa_K256014.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             19..769
                     /label=BBa_I15008
     misc_feature    782..785
                     /label=conserved
     RBS             778..789
                     /label=BBa_B0034
     CDS             796..1515
                     /label=GFP protein
     CDS             796..1515
                     /label=BBa_E0040
     stem_loop       1535..1578
                     /label=stem_loop
     misc_feature    1524..1603
                     /label=BBa_B0010
     terminator      1524..1603
                     /label=BBa_B0010
     stem_loop       1619..1638
                     /label=T7 TE
     misc_feature    1645..1645
                     /label=stop
     polyA_site      1639..1652
                     /label=polya
     misc_feature    1612..1652
                     /label=BBa_B0012
     terminator      1612..1652
                     /label=BBa_B0012
     terminator      1524..1652
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gagtgtcaac ttagcttccc agttgcggga agggacgaaa
       61 aaatcccact ccatggcgga gaacgtcggc tttgtcaaat gcttcctcaa gggcgttgtc
      121 gagaaaaatt cctaccgtaa gctggttggc aatctctact ttgtctacag tgccatggaa
      181 gaggaaatgg caaaatttaa ggaccatccc atcctcagcc acatttactt ccccgaactc
      241 aaccgcaaac aaagcctaga gcaagacctg caattctatt acggctccaa ctggcggcaa
      301 gaagtgaaaa tttctgccgc tggccaagcc tatgtggacc gagtccggca agtggccgct
      361 acggcccctg aattgttggt ggcccattcc tacacccgtt acctggggga tctttccggc
      421 ggtcaaattc tcaagaaaat tgcccaaaat gccatgaatc tccacgatgg tggcacagct
      481 ttctatgaat ttgccgacat tgatgacgaa aaggctttta aaaataccta ccgtcaagct
      541 atgaatgatc tgcccattga ccaagccacc gccgaacgga ttgtggatga agccaatgac
      601 gcctttgcca tgaacatgaa aatgttcaac gaacttgaag gcaacctgat caaggcgatc
      661 ggcattatgg tgttcaacag cctcacccgt cgccgcagtc aaggcagcac cgaagttggc
      721 ctcgccacct ccgaaggcta ataacgctga tagtgctagt gtagatcgct actagagaaa
      781 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      841 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      901 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      961 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     1021 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     1081 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     1141 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     1201 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     1261 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     1321 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     1381 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1441 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     1501 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1561 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1621 ctcaccttcg ggtgggcctt tctgcgttta ta
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