LOCUS       BBa_K256016             1870 bp    DNA     linear       06-Nov-2024
DEFINITION  B0034:IFP:B0034:GFP :B0015
ACCESSION   BBa_K256016
VERSION     BBa_K256016.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..987
                     /label=misc
     CDS             19..987
                     /label=BBa_K256008
     misc_feature    1000..1003
                     /label=conserved
     RBS             996..1007
                     /label=BBa_B0034
     CDS             1014..1733
                     /label=GFP protein
     CDS             1014..1733
                     /label=BBa_E0040
     stem_loop       1753..1796
                     /label=stem_loop
     misc_feature    1742..1821
                     /label=BBa_B0010
     terminator      1742..1821
                     /label=BBa_B0010
     stem_loop       1837..1856
                     /label=T7 TE
     misc_feature    1863..1863
                     /label=stop
     polyA_site      1857..1870
                     /label=polya
     misc_feature    1830..1870
                     /label=BBa_B0012
     terminator      1830..1870
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggcacgtgat ccgctgccgt tttttcctcc gctgtatctg
       61 ggtggtccgg aaattaccac cgaaaattgt gaacgcgaac cgattcatat tccgggtagc
      121 attcagccgc atggtgcact gctgaccgca gatggtcata gcggtgaagt tctgcaagtt
      181 agcctgaatg cagcaacctt tctgggtcag gaaccgaccg ttctgcgcgg tcagaccctg
      241 gccgcactgc tgccggaaca gtggcctgca ctgcaagcag ctctgcctcc gggttgtccg
      301 gatgcactgc aatatcgtgc aaccctggat tggccagcag caggtcatct gagcctgacc
      361 gttcatcgtg ttgcagaact gctgattctg gaatttgaac cgaccgaagc atgggatagc
      421 attggtccgc atgcgctgcg taatgcaatg tttgcactgg aaagcgcacc gaatctgcgt
      481 gcactggcag aagttgcaac ccagaccgtt cgtgaactga ccggttttga tcgtgtgatg
      541 ctgtataaat ttgccccgga tgcaaccggt gaaatgattg cagaagcacg tcgcgaaggt
      601 atgcaggcat ttctgggtca tcgttttccg gcaagccata caccggcaca ggcccgtgca
      661 ctgtataccc gtcatctgct gcgtctgacc gctgataccc gtgcagcagc agttccgctg
      721 gatccggttc tgaatccgca gaccaatgca ccgacaccgc tgggtggtgc agttctgcgt
      781 gcaaccagcc cgatgcatat gcagtatctg cgtaatatgg gtgttggtag cagcctgagc
      841 gttagcgttg ttgttggtgg tcagctgtgg ggtctgattg tttgtcatca tcagaccccg
      901 tatgttctgc cgccggatct gcgtaccacc ctggaagaac tgggtcgtaa actgagcggt
      961 caggttcagc gtaaagaagc ataataatac tagagaaaga ggagaaatac tagatgcgta
     1021 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     1081 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     1141 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     1201 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     1261 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1321 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     1381 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1441 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     1501 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     1561 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1621 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1681 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1741 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1801 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1861 tgcgtttata
//