LOCUS       BBa_K256017             1913 bp    DNA     linear       16-Dec-2024
DEFINITION  J23119:IFP:GFP
ACCESSION   BBa_K256017
VERSION     BBa_K256017.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..1030
                     /label=misc
     CDS             62..1030
                     /label=BBa_K256008
     misc_feature    1043..1046
                     /label=conserved
     RBS             1039..1050
                     /label=BBa_B0034
     CDS             1057..1776
                     /label=GFP protein
     CDS             1057..1776
                     /label=BBa_E0040
     stem_loop       1796..1839
                     /label=stem_loop
     misc_feature    1785..1864
                     /label=BBa_B0010
     terminator      1785..1864
                     /label=BBa_B0010
     stem_loop       1880..1899
                     /label=T7 TE
     misc_feature    1906..1906
                     /label=stop
     polyA_site      1900..1913
                     /label=polya
     misc_feature    1873..1913
                     /label=BBa_B0012
     terminator      1873..1913
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatggcacgt gatccgctgc cgttttttcc tccgctgtat ctgggtggtc cggaaattac
      121 caccgaaaat tgtgaacgcg aaccgattca tattccgggt agcattcagc cgcatggtgc
      181 actgctgacc gcagatggtc atagcggtga agttctgcaa gttagcctga atgcagcaac
      241 ctttctgggt caggaaccga ccgttctgcg cggtcagacc ctggccgcac tgctgccgga
      301 acagtggcct gcactgcaag cagctctgcc tccgggttgt ccggatgcac tgcaatatcg
      361 tgcaaccctg gattggccag cagcaggtca tctgagcctg accgttcatc gtgttgcaga
      421 actgctgatt ctggaatttg aaccgaccga agcatgggat agcattggtc cgcatgcgct
      481 gcgtaatgca atgtttgcac tggaaagcgc accgaatctg cgtgcactgg cagaagttgc
      541 aacccagacc gttcgtgaac tgaccggttt tgatcgtgtg atgctgtata aatttgcccc
      601 ggatgcaacc ggtgaaatga ttgcagaagc acgtcgcgaa ggtatgcagg catttctggg
      661 tcatcgtttt ccggcaagcc atacaccggc acaggcccgt gcactgtata cccgtcatct
      721 gctgcgtctg accgctgata cccgtgcagc agcagttccg ctggatccgg ttctgaatcc
      781 gcagaccaat gcaccgacac cgctgggtgg tgcagttctg cgtgcaacca gcccgatgca
      841 tatgcagtat ctgcgtaata tgggtgttgg tagcagcctg agcgttagcg ttgttgttgg
      901 tggtcagctg tggggtctga ttgtttgtca tcatcagacc ccgtatgttc tgccgccgga
      961 tctgcgtacc accctggaag aactgggtcg taaactgagc ggtcaggttc agcgtaaaga
     1021 agcataataa tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1081 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1141 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1201 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1261 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1321 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1381 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1441 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1501 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1561 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1621 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1681 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1741 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     1801 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1861 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//