BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K256009
1
BBa_K256009
IFP:B0015
2009-10-16T11:00:00Z
2015-05-08T01:11:41Z
--
Intermediate of IFP(infrared protein) Generator
false
false
_359_
0
4864
9
It's complicated
false
--
false
iGEM09_NTU-Singapore
component2048380
1
BBa_K256008
component2048381
1
BBa_B0010
component2048383
1
BBa_B0012
annotation2048380
1
BBa_K256008
range2048380
1
1
969
annotation2048383
1
BBa_B0012
range2048383
1
1066
1106
annotation2048381
1
BBa_B0010
range2048381
1
978
1057
BBa_K256022
1
BBa_K256022
J23119:IFP:HO1
2009-10-18T11:00:00Z
2015-05-08T01:11:41Z
--
Generator of IFP (Infrared protein) and HO1 (Heme Oxygenase)
false
false
_359_
0
4864
9
Not in stock
false
--
false
iGEM09_NTU-Singapore
component2276935
1
BBa_J23119
component2276937
1
BBa_B0034
component2276945
1
BBa_K256009
component2276956
1
BBa_B0015
component2276949
1
BBa_I15008
component2276947
1
BBa_B0034
annotation2276956
1
BBa_B0015
range2276956
1
1953
2081
annotation2276949
1
BBa_I15008
range2276949
1
1194
1944
annotation2276935
1
BBa_J23119
range2276935
1
1
35
annotation2276945
1
BBa_K256009
range2276945
1
62
1167
annotation2276937
1
BBa_B0034
range2276937
1
44
55
annotation2276947
1
BBa_B0034
range2276947
1
1176
1187
BBa_K256008
1
IFP
Infrared Protein (IFP 1.4)
2009-10-16T11:00:00Z
2015-05-08T01:11:41Z
Tsien's lab
Sequence from Tsien's lab; Code for the expression of Infrared protein; must co-express with Heme Oxygenase-1 (HO1) to produce infrared signal
false
false
_359_
0
4864
9
Not in stock
true
--
false
iGEM09_NTU-Singapore
annotation2056817
1
misc
range2056817
1
1
969
BBa_I15008
1
BBa_I15008
heme oxygenase (ho1) from Synechocystis
2004-09-19T11:00:00Z
2015-08-31T04:07:38Z
Voigt Lab via Anselm Levskaya
Released HQ 2013
One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
false
false
_5_
0
88
7
In stock
true
true
Jeff Tabor
annotation2214026
1
Help:Barcodes
range2214026
1
727
751
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K256008_sequence
1
atggcacgtgatccgctgccgttttttcctccgctgtatctgggtggtccggaaattaccaccgaaaattgtgaacgcgaaccgattcatattccgggtagcattcagccgcatggtgcactgctgaccgcagatggtcatagcggtgaagttctgcaagttagcctgaatgcagcaacctttctgggtcaggaaccgaccgttctgcgcggtcagaccctggccgcactgctgccggaacagtggcctgcactgcaagcagctctgcctccgggttgtccggatgcactgcaatatcgtgcaaccctggattggccagcagcaggtcatctgagcctgaccgttcatcgtgttgcagaactgctgattctggaatttgaaccgaccgaagcatgggatagcattggtccgcatgcgctgcgtaatgcaatgtttgcactggaaagcgcaccgaatctgcgtgcactggcagaagttgcaacccagaccgttcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgccccggatgcaaccggtgaaatgattgcagaagcacgtcgcgaaggtatgcaggcatttctgggtcatcgttttccggcaagccatacaccggcacaggcccgtgcactgtatacccgtcatctgctgcgtctgaccgctgatacccgtgcagcagcagttccgctggatccggttctgaatccgcagaccaatgcaccgacaccgctgggtggtgcagttctgcgtgcaaccagcccgatgcatatgcagtatctgcgtaatatgggtgttggtagcagcctgagcgttagcgttgttgttggtggtcagctgtggggtctgattgtttgtcatcatcagaccccgtatgttctgccgccggatctgcgtaccaccctggaagaactgggtcgtaaactgagcggtcaggttcagcgtaaagaagcataataa
BBa_K256022_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggcacgtgatccgctgccgttttttcctccgctgtatctgggtggtccggaaattaccaccgaaaattgtgaacgcgaaccgattcatattccgggtagcattcagccgcatggtgcactgctgaccgcagatggtcatagcggtgaagttctgcaagttagcctgaatgcagcaacctttctgggtcaggaaccgaccgttctgcgcggtcagaccctggccgcactgctgccggaacagtggcctgcactgcaagcagctctgcctccgggttgtccggatgcactgcaatatcgtgcaaccctggattggccagcagcaggtcatctgagcctgaccgttcatcgtgttgcagaactgctgattctggaatttgaaccgaccgaagcatgggatagcattggtccgcatgcgctgcgtaatgcaatgtttgcactggaaagcgcaccgaatctgcgtgcactggcagaagttgcaacccagaccgttcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgccccggatgcaaccggtgaaatgattgcagaagcacgtcgcgaaggtatgcaggcatttctgggtcatcgttttccggcaagccatacaccggcacaggcccgtgcactgtatacccgtcatctgctgcgtctgaccgctgatacccgtgcagcagcagttccgctggatccggttctgaatccgcagaccaatgcaccgacaccgctgggtggtgcagttctgcgtgcaaccagcccgatgcatatgcagtatctgcgtaatatgggtgttggtagcagcctgagcgttagcgttgttgttggtggtcagctgtggggtctgattgtttgtcatcatcagaccccgtatgttctgccgccggatctgcgtaccaccctggaagaactgggtcgtaaactgagcggtcaggttcagcgtaaagaagcataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K256009_sequence
1
atggcacgtgatccgctgccgttttttcctccgctgtatctgggtggtccggaaattaccaccgaaaattgtgaacgcgaaccgattcatattccgggtagcattcagccgcatggtgcactgctgaccgcagatggtcatagcggtgaagttctgcaagttagcctgaatgcagcaacctttctgggtcaggaaccgaccgttctgcgcggtcagaccctggccgcactgctgccggaacagtggcctgcactgcaagcagctctgcctccgggttgtccggatgcactgcaatatcgtgcaaccctggattggccagcagcaggtcatctgagcctgaccgttcatcgtgttgcagaactgctgattctggaatttgaaccgaccgaagcatgggatagcattggtccgcatgcgctgcgtaatgcaatgtttgcactggaaagcgcaccgaatctgcgtgcactggcagaagttgcaacccagaccgttcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgccccggatgcaaccggtgaaatgattgcagaagcacgtcgcgaaggtatgcaggcatttctgggtcatcgttttccggcaagccatacaccggcacaggcccgtgcactgtatacccgtcatctgctgcgtctgaccgctgatacccgtgcagcagcagttccgctggatccggttctgaatccgcagaccaatgcaccgacaccgctgggtggtgcagttctgcgtgcaaccagcccgatgcatatgcagtatctgcgtaatatgggtgttggtagcagcctgagcgttagcgttgttgttggtggtcagctgtggggtctgattgtttgtcatcatcagaccccgtatgttctgccgccggatctgcgtaccaccctggaagaactgggtcgtaaactgagcggtcaggttcagcgtaaagaagcataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I15008_sequence
1
atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z