BBa_K256024 1 BBa_K256024 CHE:B0015 2009-10-18T11:00:00Z 2015-05-08T01:11:41Z -- Intermediate of CHE(cholesterol esterase) Generator. false false _359_ 0 4864 9 Not in stock false -- false iGEM09_NTU-Singapore component2052719 1 BBa_K256023 component2052722 1 BBa_B0012 component2052720 1 BBa_B0010 annotation2052722 1 BBa_B0012 range2052722 1 1039 1079 annotation2052720 1 BBa_B0010 range2052720 1 951 1030 annotation2052719 1 BBa_K256023 range2052719 1 1 942 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K256023 1 CHE Cholesteryl Esterase (CHE) 2009-10-16T11:00:00Z 2015-05-08T01:11:41Z -- From Pseudomonas aeruginosa; Code for expression of cholesterol esterase (CHE)which convert cholesteryl ester to free cholesterol false false _359_ 0 4864 9 Not in stock true -- false Zhu Meng annotation2056824 1 misc range2056824 1 1 942 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K256023_sequence 1 atgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataa BBa_K256024_sequence 1 atgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z