BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K256023 1 CHE Cholesteryl Esterase (CHE) 2009-10-16T11:00:00Z 2015-05-08T01:11:41Z -- From Pseudomonas aeruginosa; Code for expression of cholesterol esterase (CHE)which convert cholesteryl ester to free cholesterol false false _359_ 0 4864 9 Not in stock true -- false Zhu Meng annotation2056824 1 misc range2056824 1 1 942 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K256025 1 BBa_K256025 B0034:CHE:B0015 2009-10-18T11:00:00Z 2015-05-08T01:11:41Z -- Intermediate of CHE(Cholesterol esterase) generator false false _359_ 0 4864 9 Not in stock false -- false iGEM09_NTU-Singapore component2052705 1 BBa_K256023 component2052706 1 BBa_B0010 component2052708 1 BBa_B0012 component2052704 1 BBa_B0034 annotation2052704 1 BBa_B0034 range2052704 1 1 12 annotation2052705 1 BBa_K256023 range2052705 1 19 960 annotation2052708 1 BBa_B0012 range2052708 1 1057 1097 annotation2052706 1 BBa_B0010 range2052706 1 969 1048 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K256023_sequence 1 atgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K256025_sequence 1 aaagaggagaaatactagatgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z