BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K256027
1
BBa_K256027
B0034:CHE:B0034:GFP:B0015
2009-10-18T11:00:00Z
2015-05-08T01:11:41Z
--
Intermediate of CHE , GFP generator.
false
false
_359_
0
4864
9
Not in stock
false
--
false
iGEM09_NTU-Singapore
component2052856
1
BBa_K256023
component2052855
1
BBa_B0034
component2052860
1
BBa_E0040
component2052863
1
BBa_B0012
component2052858
1
BBa_B0034
component2052861
1
BBa_B0010
annotation2052858
1
BBa_B0034
range2052858
1
969
980
annotation2052855
1
BBa_B0034
range2052855
1
1
12
annotation2052860
1
BBa_E0040
range2052860
1
987
1706
annotation2052863
1
BBa_B0012
range2052863
1
1803
1843
annotation2052861
1
BBa_B0010
range2052861
1
1715
1794
annotation2052856
1
BBa_K256023
range2052856
1
19
960
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K256023
1
CHE
Cholesteryl Esterase (CHE)
2009-10-16T11:00:00Z
2015-05-08T01:11:41Z
--
From Pseudomonas aeruginosa; Code for expression of cholesterol esterase (CHE)which convert cholesteryl ester to free cholesterol
false
false
_359_
0
4864
9
Not in stock
true
--
false
Zhu Meng
annotation2056824
1
misc
range2056824
1
1
942
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K256023_sequence
1
atgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K256027_sequence
1
aaagaggagaaatactagatgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z