BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K256032 1 BBa_K256032 placiq:luxR:pluxR:CHE 2009-10-18T11:00:00Z 2015-05-08T01:11:41Z -- CHE (Cholesterol esterase) generator. Inducible by AHL false false _359_ 0 4864 9 Not in stock false -- false iGEM09_NTU-Singapore component2288102 1 BBa_B0015 component2288118 1 BBa_B0015 component2288089 1 BBa_B0034 component2288111 1 BBa_K256023 component2288087 1 BBa_I14032 component2288109 1 BBa_B0034 component2288104 1 BBa_R0062 component2288092 1 BBa_C0062 annotation2288118 1 BBa_B0015 range2288118 1 2021 2149 annotation2288087 1 BBa_I14032 range2288087 1 1 37 annotation2288089 1 BBa_B0034 range2288089 1 46 57 annotation2288109 1 BBa_B0034 range2288109 1 1053 1064 annotation2288102 1 BBa_B0015 range2288102 1 853 981 annotation2288092 1 BBa_C0062 range2288092 1 64 819 annotation2288111 1 BBa_K256023 range2288111 1 1071 2012 annotation2288104 1 BBa_R0062 range2288104 1 990 1044 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028342 1 P(Lac) IQ range1028342 1 1 37 annotation1028344 1 -10 range1028344 1 26 31 annotation1028343 1 -35 range1028343 1 3 8 BBa_K256023 1 CHE Cholesteryl Esterase (CHE) 2009-10-16T11:00:00Z 2015-05-08T01:11:41Z -- From Pseudomonas aeruginosa; Code for expression of cholesterol esterase (CHE)which convert cholesteryl ester to free cholesterol false false _359_ 0 4864 9 Not in stock true -- false Zhu Meng annotation2056824 1 misc range2056824 1 1 942 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation1765 1 A range1765 1 492 492 annotation7039 1 BBa_C0062 range7039 1 1 756 annotation1762 1 prefix range1762 1 1 2 annotation1766 1 luxR range1766 1 1 750 annotation1764 1 T range1764 1 174 174 annotation2213986 1 Help:Barcodes range2213986 1 757 781 BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2048 1 start range2048 1 53 53 annotation2047 1 -10 range2047 1 42 47 annotation2046 1 -35 range2046 1 20 25 annotation2045 1 LuxR/HSL range2045 1 1 20 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_K256023_sequence 1 atgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_K256032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaaaaaaaaaagcctgctgccgctgggtctggcaattggtctggcaagcctggcagcatctccgctgattcaggcaagcacctatacccagaccaaatatccgattgttctggcacatggtatgctgggctttgataatattctgggcgtggattattggtttggtattccgagcgcactgcgtcgtgatggtgcacaggtttatgttaccgaagttagccagctggataccagcgaagttcgtggtgaacagctgctgcaacaggttgaagaaattgttgcactgagcggtcagccgaaagttaatctgattggtcatagccatggtggtccgaccattcgttatgttgcagcagttcgtccggatctgattgcaagcgcaaccagcgttggtgcaccgcataaaggtagcgataccgcagattttctgcgtcagattcctccgggtagcgcaggcgaagcaattctgagcggtctggttaatagcctgggtgcactgattagctttctgagcagcggtagcaccggcacccagaatagtctgggttctctggaaagcctgaatagcgaaggtgcagcacgttttaatgcaaaatatccgcagggtgttccgaccagcgcatgtggtgaaggtgcctataaagttaatggcgtgagctattatagctggtctggtagctctccgctgaccaattttctggatccgtctgatgcatttctgggtgcaagcagcctgacctttaaaaatggcaccgcaaatgatggtctggttggcacctgtagcagccatctgggtatggttattcgcgataattaccgcatgaatcatctggatgaagtgaatcaggtttttggtctgaccagcctgtttgaaacctctccggttagcgtttatcgtcagcatgcaaatcgtctgaaaaatgccagcctgtaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z