BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K256018 1 BBa_K256018 J23119:IFP 2009-10-16T11:00:00Z 2015-05-08T01:11:41Z -- IFP Generator false false _359_ 0 4864 9 It's complicated true -- false iGEM09_NTU-Singapore component2048511 1 BBa_K256008 component2048512 1 BBa_B0010 component2048514 1 BBa_B0012 component2048510 1 BBa_B0034 component2048508 1 BBa_J23119 annotation2048508 1 BBa_J23119 range2048508 1 1 35 annotation2048514 1 BBa_B0012 range2048514 1 1127 1167 annotation2048510 1 BBa_B0034 range2048510 1 44 55 annotation2048512 1 BBa_B0010 range2048512 1 1039 1118 annotation2048511 1 BBa_K256008 range2048511 1 62 1030 BBa_K256008 1 IFP Infrared Protein (IFP 1.4) 2009-10-16T11:00:00Z 2015-05-08T01:11:41Z Tsien's lab Sequence from Tsien's lab; Code for the expression of Infrared protein; must co-express with Heme Oxygenase-1 (HO1) to produce infrared signal false false _359_ 0 4864 9 Not in stock true -- false iGEM09_NTU-Singapore annotation2056817 1 misc range2056817 1 1 969 BBa_I15008 1 BBa_I15008 heme oxygenase (ho1) from Synechocystis 2004-09-19T11:00:00Z 2015-08-31T04:07:38Z Voigt Lab via Anselm Levskaya Released HQ 2013 One of two requisite genes required for the biosynthesis of phycocyanobilin from heme. false false _5_ 0 88 7 In stock true true Jeff Tabor annotation2214026 1 Help:Barcodes range2214026 1 727 751 BBa_K256033 1 BBa_K256033 Infrared signal reporter (J23119:IFP:J23119:HO1) 2009-10-19T11:00:00Z 2015-05-08T01:11:41Z Gene sequence is obtained directly from Tsien's lab. This part co-expresses IFP and HO1. false false _359_ 0 4864 9 It's complicated true -- false iGEM09_NTU-Singapore component2288200 1 BBa_K256018 component2288213 1 BBa_K256020 annotation2288213 1 BBa_K256020 range2288213 1 1176 2124 annotation2288200 1 BBa_K256018 range2288200 1 1 1167 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K256020 1 BBa_K256020 J23119:HO1 2009-10-18T11:00:00Z 2015-05-08T01:11:41Z -- Generator of Heme Oxygenase (HO1) false false _359_ 0 4864 9 It's complicated true -- false iGEM09_NTU-Singapore component2256399 1 BBa_B0015 component2256392 1 BBa_I15008 component2256390 1 BBa_B0034 component2256388 1 BBa_J23119 annotation2256392 1 BBa_I15008 range2256392 1 62 812 annotation2256388 1 BBa_J23119 range2256388 1 1 35 annotation2256390 1 BBa_B0034 range2256390 1 44 55 annotation2256399 1 BBa_B0015 range2256399 1 821 949 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K256008_sequence 1 atggcacgtgatccgctgccgttttttcctccgctgtatctgggtggtccggaaattaccaccgaaaattgtgaacgcgaaccgattcatattccgggtagcattcagccgcatggtgcactgctgaccgcagatggtcatagcggtgaagttctgcaagttagcctgaatgcagcaacctttctgggtcaggaaccgaccgttctgcgcggtcagaccctggccgcactgctgccggaacagtggcctgcactgcaagcagctctgcctccgggttgtccggatgcactgcaatatcgtgcaaccctggattggccagcagcaggtcatctgagcctgaccgttcatcgtgttgcagaactgctgattctggaatttgaaccgaccgaagcatgggatagcattggtccgcatgcgctgcgtaatgcaatgtttgcactggaaagcgcaccgaatctgcgtgcactggcagaagttgcaacccagaccgttcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgccccggatgcaaccggtgaaatgattgcagaagcacgtcgcgaaggtatgcaggcatttctgggtcatcgttttccggcaagccatacaccggcacaggcccgtgcactgtatacccgtcatctgctgcgtctgaccgctgatacccgtgcagcagcagttccgctggatccggttctgaatccgcagaccaatgcaccgacaccgctgggtggtgcagttctgcgtgcaaccagcccgatgcatatgcagtatctgcgtaatatgggtgttggtagcagcctgagcgttagcgttgttgttggtggtcagctgtggggtctgattgtttgtcatcatcagaccccgtatgttctgccgccggatctgcgtaccaccctggaagaactgggtcgtaaactgagcggtcaggttcagcgtaaagaagcataataa BBa_K256033_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggcacgtgatccgctgccgttttttcctccgctgtatctgggtggtccggaaattaccaccgaaaattgtgaacgcgaaccgattcatattccgggtagcattcagccgcatggtgcactgctgaccgcagatggtcatagcggtgaagttctgcaagttagcctgaatgcagcaacctttctgggtcaggaaccgaccgttctgcgcggtcagaccctggccgcactgctgccggaacagtggcctgcactgcaagcagctctgcctccgggttgtccggatgcactgcaatatcgtgcaaccctggattggccagcagcaggtcatctgagcctgaccgttcatcgtgttgcagaactgctgattctggaatttgaaccgaccgaagcatgggatagcattggtccgcatgcgctgcgtaatgcaatgtttgcactggaaagcgcaccgaatctgcgtgcactggcagaagttgcaacccagaccgttcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgccccggatgcaaccggtgaaatgattgcagaagcacgtcgcgaaggtatgcaggcatttctgggtcatcgttttccggcaagccatacaccggcacaggcccgtgcactgtatacccgtcatctgctgcgtctgaccgctgatacccgtgcagcagcagttccgctggatccggttctgaatccgcagaccaatgcaccgacaccgctgggtggtgcagttctgcgtgcaaccagcccgatgcatatgcagtatctgcgtaatatgggtgttggtagcagcctgagcgttagcgttgttgttggtggtcagctgtggggtctgattgtttgtcatcatcagaccccgtatgttctgccgccggatctgcgtaccaccctggaagaactgggtcgtaaactgagcggtcaggttcagcgtaaagaagcataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K256018_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggcacgtgatccgctgccgttttttcctccgctgtatctgggtggtccggaaattaccaccgaaaattgtgaacgcgaaccgattcatattccgggtagcattcagccgcatggtgcactgctgaccgcagatggtcatagcggtgaagttctgcaagttagcctgaatgcagcaacctttctgggtcaggaaccgaccgttctgcgcggtcagaccctggccgcactgctgccggaacagtggcctgcactgcaagcagctctgcctccgggttgtccggatgcactgcaatatcgtgcaaccctggattggccagcagcaggtcatctgagcctgaccgttcatcgtgttgcagaactgctgattctggaatttgaaccgaccgaagcatgggatagcattggtccgcatgcgctgcgtaatgcaatgtttgcactggaaagcgcaccgaatctgcgtgcactggcagaagttgcaacccagaccgttcgtgaactgaccggttttgatcgtgtgatgctgtataaatttgccccggatgcaaccggtgaaatgattgcagaagcacgtcgcgaaggtatgcaggcatttctgggtcatcgttttccggcaagccatacaccggcacaggcccgtgcactgtatacccgtcatctgctgcgtctgaccgctgatacccgtgcagcagcagttccgctggatccggttctgaatccgcagaccaatgcaccgacaccgctgggtggtgcagttctgcgtgcaaccagcccgatgcatatgcagtatctgcgtaatatgggtgttggtagcagcctgagcgttagcgttgttgttggtggtcagctgtggggtctgattgtttgtcatcatcagaccccgtatgttctgccgccggatctgcgtaccaccctggaagaactgggtcgtaaactgagcggtcaggttcagcgtaaagaagcataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K256020_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I15008_sequence 1 atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z