##gff-version 3
##sequence-region BBa_K257004 1 749
BBa_K257004	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_K257004	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K257004	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K257004	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K257004	.	engineered_region	46	749	.	+	0	ID=BBa_K136050;Name=BBa_K136050
BBa_K257004	.	CDS	65	749	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K136050
BBa_K257004	.	tag	685	718	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K257004	.	engineered_tag	725	749	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K257004	.	CDS	68	684	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K257004	.	ribosome_entry_site	46	58	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K136050
BBa_K257004	.	engineered_region	46	58	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K257004	.	ribosome_entry_site	46	58	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K257004	.	ribosome_entry_site	52	55	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_K257004
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagtcacacaggaaagtactagatgtccagattagat
aaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccc
agaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattga
gatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaa
agttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatg
aaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgt
ggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactact
gatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcc
ttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttt
agtagcttaataacactgatagtgctagtgtagatcac