LOCUS       BBa_K257010             1620 bp    DNA     linear       25-Nov-2024
DEFINITION  clyA + RFP
ACCESSION   BBa_K257010
VERSION     BBa_K257010.1  
FEATURES             Location/Qualifiers
     RBS             1..8
                     /label=Good rbs
     CDS             13..921
                     /label=hlyE
     misc_feature    922..936
                     /label=poly Gly linker
     misc_feature    1..937
                     /label=BBa_K257002
     CDS             943..1614
                     /label=RFP
     misc_feature    1615..1620
                     /label=double codon stop
     misc_feature    938..1620
                     /label=BBa_K257008
ORIGIN
        1 aggaggtata taatgactga aatcgttgca gataaaacgg tagaagtagt taaaaacgca
       61 atcgaaaccg cagatggagc attagatctt tataataaat atctcgatca ggtcatcccc
      121 tggcagacct ttgatgaaac cataaaagag ttaagtcgct ttaaacagga gtattcacag
      181 gcagcctccg ttttagtcgg cgatattaaa accttactta tggatagcca ggataagtat
      241 tttgaagcaa cccaaacagt gtatgaatgg tgtggtgttg cgacgcaatt gctcgcagcg
      301 tatattttgc tatttgatga gtacaatgag aagaaagcat ccgcccagaa agacattctc
      361 attaaggtac tggatgacgg catcacgaag ctgaatgaag cgcaaaaatc cctgctggta
      421 agctcacaaa gtttcaacaa cgcttccggg aaactgctgg cgttagatag ccagttaacc
      481 aatgattttt cagaaaaaag cagctatttc cagtcacagg tagataaaat caggaaggaa
      541 gcatatgccg gtgccgcagc cggtgtcgtc gccggtccat ttggattaat catttcctat
      601 tctattgctg cgggcgtagt tgaaggaaaa ctgattccag aattgaagaa caagttaaaa
      661 tctgtgcaga atttctttac caccctgtct aacacggtta aacaagcgaa taaagatatc
      721 gatgccgcca aattgaaatt aaccaccgaa atagccgcca tcggtgagat aaaaacggaa
      781 actgaaacaa ccagattcta cgttgattat gatgatttaa tgctttcttt gctaaaagaa
      841 gcggccaaaa aaatgattaa cacctgtaat gagtatcaga aaagacacgg taaaaagaca
      901 ctctttgagg tacctgaagt cggcggcggc ggcggcacta gagcttcctc cgaagacgtt
      961 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
     1021 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
     1081 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
     1141 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
     1201 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
     1261 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
     1321 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
     1381 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
     1441 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
     1501 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
     1561 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
//