BBa_K257011 1 PBad-clyA- PBad + clyA_CTerm + RFP_NTerm 2009-10-16T11:00:00Z 2015-05-08T01:11:41Z It's a composite part. PBad comes from the registry (2008 distribution). ClyA_CTerm :: RFP_NTerm is the BBa_K257010. This part allows us to express the fusion protein clyA-RFP. In a mutant strain which constitutively produce vesicles, the fusion protein inserted in the outer membrane of the bacteria will be incorporated, during vesiculation, into the lumen of the vesicles. The promoter pBad is inductible with L-arabinose. false false _354_ 0 5130 9 It's complicated false The fusion protein is designed following the standard 23 in order to avoid frameshift in the sequence. false Soufiane Boumahdi component2057501 1 BBa_I0500 component2057509 1 BBa_K257010 annotation2057509 1 BBa_K257010 range2057509 1 1219 2838 annotation2057501 1 BBa_I0500 range2057501 1 1 1210 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498843 1 AraC range1498843 1 1 1079 annotation1498841 1 Pbad range1498841 1 1080 1210 BBa_K257010 1 clyA_RFP clyA + RFP 2009-06-19T11:00:00Z 2015-05-08T01:11:41Z i just made it up this part is just a try false false _354_ 0 5366 9 It's complicated false this is just a random sequence false Vicard Du annotation2057421 1 BBa_K257002 range2057421 1 1 937 annotation2057418 1 Good rbs range2057418 1 1 8 annotation2057424 1 BBa_K257008 range2057424 1 938 1620 annotation2057423 1 double codon stop range2057423 1 1615 1620 annotation2057419 1 hlyE range2057419 1 13 921 annotation2057420 1 poly Gly linker range2057420 1 922 936 annotation2057422 1 RFP range2057422 1 943 1614 BBa_K257010_sequence 1 aggaggtatataatgactgaaatcgttgcagataaaacggtagaagtagttaaaaacgcaatcgaaaccgcagatggagcattagatctttataataaatatctcgatcaggtcatcccctggcagacctttgatgaaaccataaaagagttaagtcgctttaaacaggagtattcacaggcagcctccgttttagtcggcgatattaaaaccttacttatggatagccaggataagtattttgaagcaacccaaacagtgtatgaatggtgtggtgttgcgacgcaattgctcgcagcgtatattttgctatttgatgagtacaatgagaagaaagcatccgcccagaaagacattctcattaaggtactggatgacggcatcacgaagctgaatgaagcgcaaaaatccctgctggtaagctcacaaagtttcaacaacgcttccgggaaactgctggcgttagatagccagttaaccaatgatttttcagaaaaaagcagctatttccagtcacaggtagataaaatcaggaaggaagcatatgccggtgccgcagccggtgtcgtcgccggtccatttggattaatcatttcctattctattgctgcgggcgtagttgaaggaaaactgattccagaattgaagaacaagttaaaatctgtgcagaatttctttaccaccctgtctaacacggttaaacaagcgaataaagatatcgatgccgccaaattgaaattaaccaccgaaatagccgccatcggtgagataaaaacggaaactgaaacaaccagattctacgttgattatgatgatttaatgctttctttgctaaaagaagcggccaaaaaaatgattaacacctgtaatgagtatcagaaaagacacggtaaaaagacactctttgaggtacctgaagtcggcggcggcggcggcactagagcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa BBa_K257011_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaggaggtatataatgactgaaatcgttgcagataaaacggtagaagtagttaaaaacgcaatcgaaaccgcagatggagcattagatctttataataaatatctcgatcaggtcatcccctggcagacctttgatgaaaccataaaagagttaagtcgctttaaacaggagtattcacaggcagcctccgttttagtcggcgatattaaaaccttacttatggatagccaggataagtattttgaagcaacccaaacagtgtatgaatggtgtggtgttgcgacgcaattgctcgcagcgtatattttgctatttgatgagtacaatgagaagaaagcatccgcccagaaagacattctcattaaggtactggatgacggcatcacgaagctgaatgaagcgcaaaaatccctgctggtaagctcacaaagtttcaacaacgcttccgggaaactgctggcgttagatagccagttaaccaatgatttttcagaaaaaagcagctatttccagtcacaggtagataaaatcaggaaggaagcatatgccggtgccgcagccggtgtcgtcgccggtccatttggattaatcatttcctattctattgctgcgggcgtagttgaaggaaaactgattccagaattgaagaacaagttaaaatctgtgcagaatttctttaccaccctgtctaacacggttaaacaagcgaataaagatatcgatgccgccaaattgaaattaaccaccgaaatagccgccatcggtgagataaaaacggaaactgaaacaaccagattctacgttgattatgatgatttaatgctttctttgctaaaagaagcggccaaaaaaatgattaacacctgtaatgagtatcagaaaagacacggtaaaaagacactctttgaggtacctgaagtcggcggcggcggcggcactagagcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z