##gff-version 3
##sequence-region BBa_K257012 1 2491
BBa_K257012	.	engineered_region	1219	2388	.	+	0	ID=BBa_J24679;Name=BBa_J24679
BBa_K257012	.	ribosome_entry_site	1219	1229	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_J24679
BBa_K257012	.	engineered_region	1219	1229	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K257012	.	ribosome_entry_site	1225	1228	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K257012	.	ribosome_entry_site	1219	1229	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K257012	.	CDS	1236	2363	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24679
BBa_K257012	.	engineered_region	1236	2363	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K257012	.	tag	2325	2363	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K257012	.	engineered_tag	2364	2388	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K257012	.	CDS	1236	2363	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K257012	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_K257012	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K257012	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K257012	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K257012	.	terminator	2397	2491	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K257012	.	terminator	2397	2437	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K257012	.	polyA_site	2424	2437	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K257012	.	engineered_region	2397	2437	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K257012	.	stop_codon	2430	2430	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K257012	.	stem_loop	2404	2423	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K257012	.	terminator	2446	2491	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K257012	.	engineered_region	2446	2491	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K257012	.	stem_loop	2458	2480	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_K257012
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagtcacacaggactactagatggtgaatgtgaaaccagtaacgttata
cgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaa
acgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaac
agtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcg
cgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcac
aatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctg
cctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaaga
cggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttct
gtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaag
gcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggt
tgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggta
gtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctgg
ggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcact
ggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg
gcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgat
agtgctagtgtagatctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagag
aatataaaaagccagattattaatccggcttttttattattt