LOCUS       BBa_K257013             3240 bp    DNA     linear       25-Nov-2024
DEFINITION  PBad + RBS_LacI_LVA + RBS + YFP + Ter
ACCESSION   BBa_K257013
VERSION     BBa_K257013.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1229
                     /label=RBS-4\Weaker
     RBS             1225..1228
                     /label=RBS
     misc_feature    1219..1229
                     /label=BBa_B0033
     RBS             1219..1229
                     /label=BBa_B0033
     misc_feature    2325..2363
                     /label=LVA
     CDS             1236..2363
                     /label=lacI-LVA
     misc_feature    1236..2363
                     /label=BBa_C0012
     misc_feature    2364..2388
                     /label=Help:Barcodes
     CDS             1236..2363
                     /label=BBa_C0012
     misc_feature    1219..2388
                     /label=BBa_J24679
     misc_feature    2401..2404
                     /label=conserved
     RBS             2397..2408
                     /label=BBa_B0034
     CDS             2415..3137
                     /label=BBa_E0030
     stem_loop       3153..3172
                     /label=T7 TE
     misc_feature    3179..3179
                     /label=stop
     polyA_site      3173..3186
                     /label=polya
     misc_feature    3146..3186
                     /label=BBa_B0012
     terminator      3146..3186
                     /label=BBa_B0012
     stem_loop       3207..3229
                     /label=stem_loop
     misc_feature    3195..3240
                     /label=BBa_B0011
     terminator      3195..3240
                     /label=BBa_B0011
     terminator      3146..3240
                     /label=BBa_B0014
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggact actagatggt gaatgtgaaa ccagtaacgt
     1261 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     1321 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     1381 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     1441 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     1501 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     1561 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     1621 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     1681 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     1741 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     1801 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     1861 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     1921 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     1981 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     2041 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     2101 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     2161 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     2221 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2281 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa
     2341 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagaaag
     2401 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2461 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2521 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2581 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
     2641 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2701 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2761 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2821 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2881 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2941 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3001 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     3061 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3121 agctgtacaa gtaataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     3181 tttatatact agagagagaa tataaaaagc cagattatta atccggcttt tttattattt
//