LOCUS BBa_K257013 3240 bp DNA linear 05-Nov-2024 DEFINITION PBad + RBS_LacI_LVA + RBS + YFP + Ter ACCESSION BBa_K257013 VERSION BBa_K257013.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1229 /label=RBS-4\Weaker RBS 1225..1228 /label=RBS misc_feature 1219..1229 /label=BBa_B0033 RBS 1219..1229 /label=BBa_B0033 misc_feature 2325..2363 /label=LVA CDS 1236..2363 /label=lacI-LVA misc_feature 1236..2363 /label=BBa_C0012 misc_feature 2364..2388 /label=Help:Barcodes CDS 1236..2363 /label=BBa_C0012 misc_feature 1219..2388 /label=BBa_J24679 misc_feature 2401..2404 /label=conserved RBS 2397..2408 /label=BBa_B0034 CDS 2415..3137 /label=BBa_E0030 stem_loop 3153..3172 /label=T7 TE misc_feature 3179..3179 /label=stop polyA_site 3173..3186 /label=polya misc_feature 3146..3186 /label=BBa_B0012 terminator 3146..3186 /label=BBa_B0012 stem_loop 3207..3229 /label=stem_loop misc_feature 3195..3240 /label=BBa_B0011 terminator 3195..3240 /label=BBa_B0011 terminator 3146..3240 /label=BBa_B0014 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggact actagatggt gaatgtgaaa ccagtaacgt 1261 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 1321 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 1381 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg 1441 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 1501 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 1561 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 1621 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 1681 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg 1741 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag 1801 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca 1861 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt 1921 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca 1981 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg 2041 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt 2101 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc 2161 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa 2221 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 2281 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa 2341 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagaaag 2401 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 2461 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 2521 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 2581 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc 2641 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 2701 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 2761 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 2821 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 2881 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 2941 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 3001 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc 3061 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 3121 agctgtacaa gtaataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3181 tttatatact agagagagaa tataaaaagc cagattatta atccggcttt tttattattt //