LOCUS       BBa_K257016             1699 bp    DNA     linear       26-Nov-2024
DEFINITION  OmpA_Linker + g3p
ACCESSION   BBa_K257016
VERSION     BBa_K257016.1  
FEATURES             Location/Qualifiers
     RBS             1..8
                     /label=good rbs
     misc_feature    1..473
                     /label=BBa_K257003
     CDS             14..448
                     /label=ompA Linker (partial)
     misc_feature    449..474
                     /label=linker for C term fusion
     misc_feature    469..474
                     /label=SacI restriction site
     misc_feature    474..1699
                     /label=BBa_K257001
     misc_feature    476..676
                     /label=N1 domain
     misc_feature    677..733
                     /label=G1 (Gly-rich linker, EGGGS motif)
     misc_feature    734..1126
                     /label=N2 domain
     misc_feature    1127..1243
                     /label=G2 (Gly-rich linker)
     misc_feature    1244..1693
                     /label=CT domain
     misc_feature    1694..1699
                     /label=Double stop codon
ORIGIN
        1 aaggaggtat ataatgaaag ctactaaact ggtactgggc gcggtaatcc tgggttctac
       61 tctgctggca ggttgctcca gcaacgctaa aatcgatcag ggaattaacc cgtatgttgg
      121 ctttgaaatg ggttacgact ggttaggtcg tatgccgtac aaaggcagcg ttgaaaacgg
      181 tgcatacaaa gctcagggcg ttcaactgac cgctaaactg ggttacccaa tcactgacga
      241 cctggacatc tacactcgtc tgggtggcat ggtatggcgt gcagacacta aatccaacgt
      301 ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc
      361 gatcactcct gaaatcgcta cccgtctgga ataccagtgg accaacaaca tcggtgacgc
      421 acacaccatc ggcactcgtc cggacaacgg cggaggttct ggaggaggga gctccgctga
      481 aactgttgaa agttgtttag caaaacccca tacagaaaat tcatttacta acgtctggaa
      541 agacgacaaa actttagatc gttacgctaa ctatgagggt tgtctgtgga atgctacagg
      601 cgttgtagtt tgtactggtg acgaaactca gtgttacggt acatgggttc ctattgggct
      661 tgctatccct gaaaatgagg gtggtggctc tgagggtggc ggttctgagg gtggcggttc
      721 tgagggtggc ggtactaaac ctcctgagta cggtgataca cctattccgg gctatactta
      781 tatcaaccct ctcgacggca cttatccgcc tggtactgag caaaaccccg ctaatcctaa
      841 tccttctctt gaggagtctc agcctcttaa tactttcatg tttcagaata ataggttccg
      901 aaataggcag ggggcattaa ctgtttatac gggcactgtt actcaaggca ctgaccccgt
      961 taaaacttat taccagtaca ctcctgtatc atcaaaagcc atgtatgacg cttactggaa
     1021 cggtaaattc agagactgcg ctttccattc tggctttaat gaggatccat tcgtttgtga
     1081 atatcaaggc caatcgtctg acctgcctca acctcctgtc aatgctggcg gcggctctgg
     1141 tggtggttct ggtggcggct ctgagggtgg tggctctgag ggtggcggtt ctgagggtgg
     1201 cggctctgag ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg attatgaaaa
     1261 gatggcaaac gctaataagg gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc
     1321 tgacgctaaa ggcaaacttg attctgtcgc tactgattac ggtgctgcta tcgatggttt
     1381 cattggtgac gtttccggcc ttgctaatgg taatggtgct actggtgatt ttgctggctc
     1441 taattcccaa atggctcaag tcggtgacgg tgataattca cctttaatga ataatttccg
     1501 tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct ttagcgctgg
     1561 taaaccatat gaattttcta ttgattgtga caaaataaac ttattccgtg gtgtctttgc
     1621 gtttctttta tatgttgcca cctttatgta tgtattttct acgtttgcta acatactgcg
     1681 taataaggag tcttaataa
//