BBa_K257016 1 OmpA-g3p OmpA_Linker + g3p 2009-10-17T11:00:00Z 2015-05-08T01:11:41Z Composite of 2 de novo part. This part allows us to send g3P to the outer membrane of the bacteria so that we can use g3P as an activator of the fusion between the vesicle and the recipient bacteria. false false _354_ 0 5130 9 It's complicated false In our project we use g3p as a fusion to OmpA-Linker (BBa_K103996) which need SacI restriction site for inframe fusion.<br> * So we design g3p with SacI site at the N-terminal. SacI (GAGCT^C) site is shared with XbaI (T^CTAGA) in order to have SacI site for fusion and standard sites.<br> * Moreover we decide to suppress the signal peptide (18 first amino acids) which is cleaved in order to conserve the N-ter fusion. false Soufiane Boumahdi annotation2057831 1 BBa_K257001 range2057831 1 474 1699 annotation2057833 1 G1 (Gly-rich linker, EGGGS motif) range2057833 1 677 733 annotation2057828 1 ompA Linker (partial) range2057828 1 14 448 annotation2057830 1 SacI restriction site range2057830 1 469 474 annotation2057837 1 Double stop codon range2057837 1 1694 1699 annotation2057826 1 good rbs range2057826 1 1 8 annotation2057827 1 BBa_K257003 range2057827 1 1 473 annotation2057835 1 G2 (Gly-rich linker) range2057835 1 1127 1243 annotation2057834 1 N2 domain range2057834 1 734 1126 annotation2057832 1 N1 domain range2057832 1 476 676 annotation2057836 1 CT domain range2057836 1 1244 1693 annotation2057829 1 linker for C term fusion range2057829 1 449 474 BBa_K257016_sequence 1 aaggaggtatataatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatccttctcttgaggagtctcagcctcttaatactttcatgtttcagaataataggttccgaaataggcagggggcattaactgtttatacgggcactgttactcaaggcactgaccccgttaaaacttattaccagtacactcctgtatcatcaaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctttccattctggctttaatgaggatccattcgtttgtgaatatcaaggccaatcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtggtggttctggtggcggctctgagggtggtggctctgagggtggcggttctgagggtggcggctctgagggaggcggttccggtggtggctctggttccggtgattttgattatgaaaagatggcaaacgctaataagggggctatgaccgaaaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgattctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtttccggccttgctaatggtaatggtgctactggtgattttgctggctctaattcccaaatggctcaagtcggtgacggtgataattcacctttaatgaataatttccgtcaatatttaccttccctccctcaatcggttgaatgtcgcccttttgtctttagcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtctttgcgtttcttttatatgttgccacctttatgtatgtattttctacgtttgctaacatactgcgtaataaggagtcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z