BBa_K257018
1
AIDA-I
AIDA-I autotransporter, linker + translocator domains
2009-10-17T11:00:00Z
2015-05-08T01:11:41Z
AIDA has been synthesized in vitro.
AIDA-I is the adhesin involved in diffuse adherence of E.coli. It belongs to the monomeric autotransporter protein family of the type-V secretion pathway. AIDA-I is synthesized from the aidA gene as a 145 kDa pre-proprotein with three domains : first, an N-terminal sec-dependent signal sequence that allows transport across the inner membrane; second, a cenral domain of 100kDa, the mature AIDA-I, bearing the functional part of the protein; and finally, a C-terminal membrane-embedded domain of 45kDa, AIDAc, which is predicted to form a pore in the outer membrane and might serve as a translocation conduit for mature AIDA-I.
false
false
_354_
0
5130
9
It's complicated
false
We had to deal with considerations about the linker domain which links the translocated protein to the translocator domain of AIDA-I. In fact, this linker needs to have a minimal length so that the protein translocated would be really functional at the outer side of the outer membrane.
false
Soufiane Boumahdi
annotation2057863
1
AIDA_I_Cterm
range2057863
1
1
1320
annotation2057928
1
Translocator domain
range2057928
1
469
1320
annotation2057864
1
double codon stop
range2057864
1
1321
1326
annotation2057927
1
Linker domain
range2057927
1
1
468
BBa_K257018_sequence
1
gcaggtaatactcttaccgtgtcaaattatactgggacaccgggaagtgttatttctcttggtggtgtgcttgaaggagataattcacttacggaccgtctggtggtgaaaggtaatacctctggtcaaagtgacatcgtttatgtcaatgaagatggcagtggtggtcagacgagagatggtattaatattatttctgtagagggaaattctgatgcagagttctctctgaagaaccgcgtagttgccggagcttatgattacacactgcaaaaaggaaacgagagtgggacagataataagggatggtatttaaccagtcatcttcccacatctgatacccggcaatacagaccggagaacggaagttatgctaccaatatggcactggctaactcactgttcctcatggatttgaatgagcgtaagcaattcagggccatgagtgataatacacagcctgagtctgcatccgtgtggatgaagatcactggaggaataagctctggtaagcttaatgacgggcaaaataaaacaacaaccaatcagtttatcaatcagctcgggggggatatttataaattccatgctgaacaactgggtgattttaccttagggattatgggaggatacgcgaatgcaaaaggtaaaacgataaattacacgagcaacaaagctgccagaaacacactggatggttattctgtcggggtatacggtacgtggtatcagaatggggaaaatgcaacagggctctttgctgaaacttggatgcaatataactggtttaatgcatcagtgaaaggtgacggactggaagaagaaaaatataatctgaatggtttaaccgcttctgccggtgggggatataacctgaatgtgcacacatggacatcacctgaaggaataacaggtgaattttggttacagcctcatttgcaggctgtctggatgggggttacaccggatacacatcaggaggataacggaacggtggtgcagggagcagggaaaaataatattcagacaaaagcaggtattcgtgcatcctggaaggtgaaaagcaccctggataaggataccgggcggaggttccgtccgtatatagaggcaaactggatccataacactcatgaatttggtgttaaaatgagtgatgacagccagttgttgtcaggtagccgaaatcagggagagataaagacaggtattgaaggggtgattactcaaaacttgtcagtgaatggcggagtcgcatatcaggcaggaggtcacgggagcaatgccatctccggagcactggggataaaatacagcttctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z