BBa_K257018 1 AIDA-I AIDA-I autotransporter, linker + translocator domains 2009-10-17T11:00:00Z 2015-05-08T01:11:41Z AIDA has been synthesized in vitro. AIDA-I is the adhesin involved in diffuse adherence of E.coli. It belongs to the monomeric autotransporter protein family of the type-V secretion pathway. AIDA-I is synthesized from the aidA gene as a 145 kDa pre-proprotein with three domains : first, an N-terminal sec-dependent signal sequence that allows transport across the inner membrane; second, a cenral domain of 100kDa, the mature AIDA-I, bearing the functional part of the protein; and finally, a C-terminal membrane-embedded domain of 45kDa, AIDAc, which is predicted to form a pore in the outer membrane and might serve as a translocation conduit for mature AIDA-I. false false _354_ 0 5130 9 It's complicated false We had to deal with considerations about the linker domain which links the translocated protein to the translocator domain of AIDA-I. In fact, this linker needs to have a minimal length so that the protein translocated would be really functional at the outer side of the outer membrane. false Soufiane Boumahdi annotation2057863 1 AIDA_I_Cterm range2057863 1 1 1320 annotation2057928 1 Translocator domain range2057928 1 469 1320 annotation2057864 1 double codon stop range2057864 1 1321 1326 annotation2057927 1 Linker domain range2057927 1 1 468 BBa_K257018_sequence 1 gcaggtaatactcttaccgtgtcaaattatactgggacaccgggaagtgttatttctcttggtggtgtgcttgaaggagataattcacttacggaccgtctggtggtgaaaggtaatacctctggtcaaagtgacatcgtttatgtcaatgaagatggcagtggtggtcagacgagagatggtattaatattatttctgtagagggaaattctgatgcagagttctctctgaagaaccgcgtagttgccggagcttatgattacacactgcaaaaaggaaacgagagtgggacagataataagggatggtatttaaccagtcatcttcccacatctgatacccggcaatacagaccggagaacggaagttatgctaccaatatggcactggctaactcactgttcctcatggatttgaatgagcgtaagcaattcagggccatgagtgataatacacagcctgagtctgcatccgtgtggatgaagatcactggaggaataagctctggtaagcttaatgacgggcaaaataaaacaacaaccaatcagtttatcaatcagctcgggggggatatttataaattccatgctgaacaactgggtgattttaccttagggattatgggaggatacgcgaatgcaaaaggtaaaacgataaattacacgagcaacaaagctgccagaaacacactggatggttattctgtcggggtatacggtacgtggtatcagaatggggaaaatgcaacagggctctttgctgaaacttggatgcaatataactggtttaatgcatcagtgaaaggtgacggactggaagaagaaaaatataatctgaatggtttaaccgcttctgccggtgggggatataacctgaatgtgcacacatggacatcacctgaaggaataacaggtgaattttggttacagcctcatttgcaggctgtctggatgggggttacaccggatacacatcaggaggataacggaacggtggtgcagggagcagggaaaaataatattcagacaaaagcaggtattcgtgcatcctggaaggtgaaaagcaccctggataaggataccgggcggaggttccgtccgtatatagaggcaaactggatccataacactcatgaatttggtgttaaaatgagtgatgacagccagttgttgtcaggtagccgaaatcagggagagataaagacaggtattgaaggggtgattactcaaaacttgtcagtgaatggcggagtcgcatatcaggcaggaggtcacgggagcaatgccatctccggagcactggggataaaatacagcttctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z