LOCUS       BBa_K257019             2341 bp    DNA     linear       23-Dec-2024
DEFINITION  FecA mutated - constitutive activity of the fec operon
ACCESSION   BBa_K257019
VERSION     BBa_K257019.1  
FEATURES             Location/Qualifiers
     RBS             1..8
                     /label=good rbs
     CDS             14..2335
                     /label=fecA
     misc_feature    2336..2341
                     /label=double codon stop
     misc_feature    374..376
                     /label=W706P
     misc_feature    287..292
                     /label=site pstI removed
ORIGIN
        1 aaggaggtat ataatgacgc cgttacgcgt ttttcgtaaa acaacacctt tggttaacac
       61 cattcgcctg agcctgctgc cgctggccgg tctctcgttt tccgcttttg ctgcacaggt
      121 taatatcgca ccgggatcgc tcgataaagc gctcaatcag tatgccgcac acagcggatt
      181 taccctctcg gttgacgcca gcctgacgcg cggcaagcag agcaacggcc tgcacggcga
      241 ttacgacgtc gagagcggcc tgcaacaact gctggacggc agcggactgc aagtaaaacc
      301 gctgggaaat aacagctgga cgctggagcc cgcgcccgca ccaaaagaag atgccctgac
      361 cgtggtcggc gactggctgg gtgatgcgcg tgaaaacgac gtatttgaac atgctggcgc
      421 gcgtgacgtg atccgccgtg aggatttcgc caaaaccggc gcaaccacca tgcgtgaggt
      481 acttaaccgc atccctggcg tcagcgcgcc ggaaaacaac ggcaccggca gccacgacct
      541 ggcgatgaac tttggcatcc ggggcctgaa cccgcgcctc gccagccgct cgaccgtcct
      601 gatggacggc atccccgtcc ccttcgcccc ttacggtcag ccgcagcttt cactggctcc
      661 cgtttcgctc ggcaacatgg atgccattga cgtggtacgc ggtggtggtg cggtgcgtta
      721 cggaccgcag agcgtgggcg gcgtggtgaa ctttgttacc cgtgccattc cgcaggactt
      781 tggtatcgag gcgggcgtgg aaggtcagct cagcccaacc tcttcacaaa acaacccgaa
      841 agagacgcac aacctgatgg tgggcggcac agcggacaac ggttttggca ccgcgctgct
      901 ctactccggc acgcgcggca gtgactggcg cgagcacagc gccacccgca tcgacgacct
      961 gatgctgaaa agcaaatatg cgccggatga ggtgcacacc ttcaacagcc tgctgcaata
     1021 ttacgacggt gaagccgaca tgcccggtgg cctgtctcgc gcggattacg acgccgatcg
     1081 ctggcaatcc acccgcccgt atgaccgctt ctggggtcgt cgcaagctgg cgagcctggg
     1141 ctaccagttc cagccagaca gccagcataa attcaacatt caggggttct acacccaaac
     1201 cctgcgcagc ggctacctgg agcaaggcaa acgcatcacc ctctcgccgc gtaactactg
     1261 ggtgcgcggt attgagccac gctacagcca gatctttatg atcggccctt ccgcgcacga
     1321 agtgggcgtg ggctatcgct atttgaatga atcaacgcat gaaatgcgtt actacaccgc
     1381 caccagcagc gggcagttgc cgtccggctc aagcccttac gaccgcgata cgcgttccgg
     1441 caccgaggcg cacgcctggt atctggatga caaaatcgac atcggcaact ggaccatcac
     1501 gccgggtatg cgtttcgaac atatcgagtc ataccagaac aacgccatca caggcacgca
     1561 cgaagaagtg agctataacg caccgcttcc ggcgttgaac gtgctctatc acctgactga
     1621 cagctggaat ctttatgcaa acactgaagg ctcgttcggc accgtacagt acagccagat
     1681 tggcaaggct gtgcaaagcg gcaatgttga accggaaaaa gcgcgaacct gggaactcgg
     1741 tacccgctac gacgacggcg cgctgacggc ggaaatgggg ctgttcctga ttaactttaa
     1801 caatcagtac gactccaacc agaccaacga caccgtcact gcacgtggca aaacgcgcca
     1861 taccgggctg gaaacgcagg cacgttacga tctgggtacg ctaacgccaa cgcttgataa
     1921 cgtttccatc tacgccagct atgcgtatgt gaacgcggaa atccgcgaga aaggcgacac
     1981 ctacggcaat ctggtaccat tctccccgaa acataaaggc acgctgggcg tggactacaa
     2041 gccaggaaac tggacgttca atctgaacag cgatttccag tccagccagt ttgcggataa
     2101 cgccaatacg gtgaaagaga gcgccgacgg cagtaccggc cgcattcccg gcttcatgct
     2161 ctggggcgca cgcgtggcgt atgactttgg cccgcagatg gcagatctga acctggcatt
     2221 cggtgtgaaa aacatcttcg accaggacta cttcatccgc tcttatgacg acaacaacaa
     2281 aggcatctat gcaggccagc cgcgcacgct gtatatgcag gggtcgttga agttctaata
     2341 a
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