##gff-version 3
##sequence-region BBa_K257021 1 1646
BBa_K257021	.	engineered_region	1	852	.	+	0	ID=BBa_K257015;Name=BBa_K257015
BBa_K257021	.	terminator	758	852	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K257015
BBa_K257021	.	terminator	807	852	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K257021	.	stem_loop	819	841	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K257021	.	engineered_region	807	852	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K257021	.	terminator	758	798	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K257021	.	stem_loop	765	784	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K257021	.	stop_codon	791	791	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K257021	.	polyA_site	785	798	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K257021	.	engineered_region	758	798	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K257021	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_K257015
BBa_K257021	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K257021	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K257021	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K257021	.	engineered_region	46	749	.	+	0	ID=BBa_K136050;Name=BBa_K136050;Parent=BBa_K257015
BBa_K257021	.	ribosome_entry_site	46	58	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K136050
BBa_K257021	.	ribosome_entry_site	46	58	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K257021	.	engineered_region	46	58	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K257021	.	ribosome_entry_site	52	55	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K257021	.	CDS	65	749	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K136050
BBa_K257021	.	engineered_tag	725	749	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K257021	.	tag	685	718	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K257021	.	CDS	68	684	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K257021	.	engineered_region	861	1646	.	+	0	ID=BBa_K257014;Name=BBa_K257014
BBa_K257021	.	engineered_region	923	1646	.	+	0	ID=BBa_K093005;Name=BBa_K093005;Parent=BBa_K257014
BBa_K257021	.	ribosome_entry_site	923	934	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_K257021	.	conserved_region	927	930	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K257021	.	CDS	941	1646	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_K257021	.	engineered_tag	1622	1646	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K257021	.	CDS	941	1615	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K257021	.	promoter	861	914	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K257014
BBa_K257021	.	non_covalent_binding_site	861	879	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K257021	.	promoter	903	908	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K257021	.	promoter	880	885	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K257021	.	non_covalent_binding_site	886	904	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K257021	.	engineered_region	861	914	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
>BBa_K257021
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagtcacacaggaaagtactagatgtccagattagat
aaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccc
agaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattga
gatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaa
agttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatg
aaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgt
ggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactact
gatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcc
ttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttt
agtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtccctatca
gtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttc
ctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatc
gaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgt
tcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggacta
cctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacc
caggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccgg
ttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaat
caaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggtt
cagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagt
acgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc