##gff-version 3
##sequence-region BBa_K257023 1 3044
BBa_K257023	.	engineered_region	733	861	.	+	0	ID=annotation2058126;Name=BBa_B0015
BBa_K257023	.	operator	2997	3002	.	+	0	ID=annotation2058140;Name=-10
BBa_K257023	.	sequence_feature	870	877	.	+	0	ID=annotation2058128;Name=NotI
BBa_K257023	.	CDS	1818	2678	.	+	0	ID=annotation2058133;Name=beta-lactamase ampicillin resistance marker
BBa_K257023	.	sequence_feature	877	882	.	+	0	ID=annotation2058129;Name=PstI
BBa_K257023	.	primer_binding_site	2801	2820	.	+	0	ID=annotation2058135;Name=Verification forward (VF2) primer binding site
BBa_K257023	.	CDS	19	699	.	+	0	ID=annotation2058125;Name=mRFP
BBa_K257023	.	primer_binding_site	940	959	.	+	0	ID=annotation2058130;Name=Verification reverse (VR) primer binding site
BBa_K257023	.	engineered_region	1	12	.	+	0	ID=annotation2058124;Name=BBa_B0034 RBS
BBa_K257023	.	sequence_feature	3011	3030	.	+	0	ID=annotation2058142;Name=important for activity
BBa_K257023	.	sequence_feature	1044	1663	.	+	0	ID=annotation2058132;Name=pBR322 origin
BBa_K257023	.	sequence_feature	1041	1723	.	+	0	ID=annotation2058131;Name=ColE1 origin
BBa_K257023	.	sequence_feature	2919	2940	.	+	0	ID=annotation2058136;Name=BioBrick prefix
BBa_K257023	.	engineered_region	1	2934	.	+	0	ID=annotation2058122;Name=BBa_J61002
BBa_K257023	.	ribosome_entry_site	1	12	.	+	0	ID=annotation2058123;Name=RBS
BBa_K257023	.	promoter	2720	2748	.	+	0	ID=annotation2058134;Name=beta-lactamase (ampR, bla) promoter
BBa_K257023	.	stem_loop	744	787	.	+	0	ID=annotation2058127;Name=rrnB terminator
BBa_K257023	.	operator	2976	2981	.	+	0	ID=annotation2058139;Name=-35
BBa_K257023	.	engineered_region	2941	3044	.	+	0	ID=annotation2058138;Name=BBa_K257000
BBa_K257023	.	sequence_alteration	2933	2933	.	+	0	ID=annotation2058137;Name=Ambiguity; should be a T
BBa_K257023	.	sequence_feature	3011	3011	.	+	0	ID=annotation2058141;Name=+1
>BBa_K257023
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc
tagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaa
gcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactca
aaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcca
ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctc
aagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct
gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgct
gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg
cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaac
aggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagga
cagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaac
caccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttg
atcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaagga
tcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacag
ttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtc
gtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccgg
ctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccat
ccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgct
acaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacat
gatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgtt
atcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgag
tactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccg
cgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgct
gttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctggg
tgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcc
tttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataa
acaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacc
tataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaatt
cgcggccgcatctagagtacgcggtactggataaacatttcaccactgtaaggaaaataattcttatttcgattgtcct
ttttacccttctcgttcgactcatagctgaacacaacaaaaa