LOCUS       BBa_K257026             1015 bp    DNA     linear       26-Nov-2024
DEFINITION  PLac + RBS-TetR + Ter
ACCESSION   BBa_K257026
VERSION     BBa_K257026.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             231..847
                     /label=tetR
     misc_feature    848..881
                     /label=SsrA
     misc_feature    888..912
                     /label=Help:Barcodes
     CDS             228..912
                     /label=BBa_C0040
     misc_feature    209..912
                     /label=BBa_K136050
     stem_loop       928..947
                     /label=T7 TE
     misc_feature    954..954
                     /label=stop
     polyA_site      948..961
                     /label=polya
     misc_feature    921..961
                     /label=BBa_B0012
     terminator      921..961
                     /label=BBa_B0012
     stem_loop       982..1004
                     /label=stem_loop
     misc_feature    970..1015
                     /label=BBa_B0011
     terminator      970..1015
                     /label=BBa_B0011
     terminator      921..1015
                     /label=BBa_B0014
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg tccagattag
      241 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
      301 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
      361 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
      421 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
      481 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
      541 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
      601 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
      661 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
      721 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
      781 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
      841 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct
      901 agtgtagatc actactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      961 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattt
//