LOCUS BBa_K257027 1809 bp DNA linear 26-Nov-2024 DEFINITION PLac + RBS-TetR + Ter + Ptet + RFP ACCESSION BBa_K257027 VERSION BBa_K257027.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 231..847 /label=tetR misc_feature 848..881 /label=SsrA misc_feature 888..912 /label=Help:Barcodes CDS 228..912 /label=BBa_C0040 misc_feature 209..912 /label=BBa_K136050 stem_loop 928..947 /label=T7 TE misc_feature 954..954 /label=stop polyA_site 948..961 /label=polya misc_feature 921..961 /label=BBa_B0012 terminator 921..961 /label=BBa_B0012 stem_loop 982..1004 /label=stem_loop misc_feature 970..1015 /label=BBa_B0011 terminator 970..1015 /label=BBa_B0011 terminator 921..1015 /label=BBa_B0014 misc_feature 1..1015 /label=BBa_K257026 misc_feature 1024..1042 /label=TetR 1 misc_feature 1024..1077 /label=BBa_R0040 promoter 1043..1048 /label=-35 misc_feature 1049..1067 /label=TetR 2 promoter 1066..1071 /label=-10 promoter 1024..1077 /label=BBa_R0040 misc_feature 1090..1093 /label=conserved RBS 1086..1097 /label=BBa_B0034 CDS 1104..1778 /label=mrfp1 misc_feature 1785..1809 /label=Help:Barcodes CDS 1104..1809 /label=BBa_E1010 misc_feature 1086..1809 /label=BBa_K093005 misc_feature 1024..1809 /label=BBa_K257014 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg tccagattag 241 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt 301 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg 361 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata 421 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa 481 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc 541 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt 601 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg 661 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata 721 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag 781 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa 841 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 901 agtgtagatc actactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 961 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattttacta 1021 gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac 1081 tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc 1141 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag 1201 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc 1261 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg 1321 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat 1381 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc 1441 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg 1501 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg 1561 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact 1621 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt 1681 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac 1741 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt 1801 gtagatcgc //