##gff-version 3
##sequence-region BBa_K257027 1 1809
BBa_K257027	.	engineered_region	1024	1809	.	+	0	ID=BBa_K257014;Name=BBa_K257014
BBa_K257027	.	engineered_region	1086	1809	.	+	0	ID=BBa_K093005;Name=BBa_K093005;Parent=BBa_K257014
BBa_K257027	.	CDS	1104	1809	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_K257027	.	engineered_tag	1785	1809	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K257027	.	CDS	1104	1778	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K257027	.	ribosome_entry_site	1086	1097	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_K257027	.	conserved_region	1090	1093	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K257027	.	promoter	1024	1077	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K257014
BBa_K257027	.	promoter	1043	1048	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K257027	.	non_covalent_binding_site	1049	1067	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K257027	.	engineered_region	1024	1077	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K257027	.	promoter	1066	1071	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K257027	.	non_covalent_binding_site	1024	1042	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K257027	.	engineered_region	1	1015	.	+	0	ID=BBa_K257026;Name=BBa_K257026
BBa_K257027	.	engineered_region	209	912	.	+	0	ID=BBa_K136050;Name=BBa_K136050;Parent=BBa_K257026
BBa_K257027	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K136050
BBa_K257027	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K257027	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K257027	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K257027	.	CDS	228	912	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K136050
BBa_K257027	.	engineered_tag	888	912	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K257027	.	tag	848	881	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K257027	.	CDS	231	847	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K257027	.	terminator	921	1015	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K257026
BBa_K257027	.	terminator	921	961	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K257027	.	engineered_region	921	961	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K257027	.	stop_codon	954	954	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K257027	.	stem_loop	928	947	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K257027	.	polyA_site	948	961	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K257027	.	terminator	970	1015	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K257027	.	engineered_region	970	1015	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K257027	.	stem_loop	982	1004	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K257027	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K257026
BBa_K257027	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K257027	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K257027	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K257027	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K257027	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K257027	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K257027	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
>BBa_K257027
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgtccagat
tagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaact
cgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcc
attgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacg
ctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaaca
gtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagc
gctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta
ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttatt
cggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactac
gctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtccc
tatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatg
gcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcg
aaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgct
gccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccg
gactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccg
ttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacgg
tccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt
gaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaac
cggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttga
acagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc