BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K258102 1 BBa_K258102 PrtF domain of ABC transporter of Erwinia chrysanthemi 2009-10-09T11:00:00Z 2015-05-08T01:11:42Z E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli. prtF, codes for a polypeptide of 462 amino acid residues (predicted Mr = 52.5 kd) that is probably initiated at an ATG codon located 15 bp downstream from the prtE gene stop codon TAA. It is preceded 11 bp upstream by a GGAAGG RBS consensus sequence.the PrtF protein is hydrophilic and analysis of the deduced amino acid sequence revealed the presence at its N-terminal end of a typical procaryotic signal peptide (italics in Figure 2) which contains three positively charged residues followed by a stretch of hydrophobic or neutral residues and ending at a consensus bacterial signal peptide cleavage site, Ala Gln Ala or Ala Met Gly. false false _358_ 0 4260 9 Not in stock false The gene actually will be fully function with other two genes which are PrtD and PrtE. false Cihan Tastan BBa_K258007 1 PrtDEF Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi 2009-10-09T11:00:00Z 2015-05-08T01:11:42Z false true _9_ 0 4260 9 It's complicated true false Cihan Tastan donated by Jung Hoon Ahn from Korea Advanced Institute of Science and Technology component2033379 1 BBa_B0034 component2033376 1 BBa_B0010 component2033369 1 BBa_B0034 component2033383 1 BBa_B0012 component2033363 1 BBa_R0040 component2033371 1 BBa_B0010 component2033374 1 BBa_B0034 component2033375 1 BBa_K258101 component2033370 1 BBa_K258100 component2033380 1 BBa_K258102 component2033381 1 BBa_B0010 annotation2033369 1 BBa_B0034 range2033369 1 63 74 annotation2033363 1 BBa_R0040 range2033363 1 1 54 annotation2033371 1 BBa_B0010 range2033371 1 1814 1893 annotation2033380 1 BBa_K258102 range2033380 1 3378 4763 annotation2033375 1 BBa_K258101 range2033375 1 1920 3263 annotation2033370 1 BBa_K258100 range2033370 1 81 1805 annotation2033383 1 BBa_B0012 range2033383 1 4860 4900 annotation2033381 1 BBa_B0010 range2033381 1 4772 4851 annotation2033379 1 BBa_B0034 range2033379 1 3360 3371 annotation2033376 1 BBa_B0010 range2033376 1 3272 3351 annotation2033374 1 BBa_B0034 range2033374 1 1902 1913 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K258101 1 BBa_K258101 PrtE domain of ABC transporter of Erwinia chrysanthemi 2009-10-09T11:00:00Z 2015-05-08T01:11:42Z E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 50 kd protein to the prtD gene product (protein PrtD). prtE, codes for a polypeptide of 448 amino acid residues (predicted Mr = 49.5 kd) which is probably initiated at an ATG codon located 14 bp downstream from the prtD gene stop codon TGA. It is preceded 11 bp upstream by a GGGA RBS consensus sequence.The hydropathy profile of the PrtE protein shows at least one hydrophobic domain with the sequence (residues 30 to 60) which might be a transmembrane segment. No signal peptide is found at the N-terminus, which is hydrophilic false false _358_ 0 4260 9 Not in stock false The gene actually will be fully function with other two genes which are PrtD and PrtF. false Cihan Tastan BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 BBa_K258100 1 BBa_K258100 PrtD domain of ABC transporter of Erwinia chrysanthemi 2009-10-09T11:00:00Z 2015-05-08T01:11:42Z E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 60 kd protein to the prtD gene product (protein PrtD). The predicted amino acid sequence of the PrtD protein indicates that this protein has a putative ATP binding site, located in the C-terminal half of the protein, which corresponds to the consensus sequence proposed for ATP binding proteins. false false _358_ 0 4260 9 Not in stock false The gene actually will be fully function with other two genes which are PrtD and PrtE false Cihan Tastan BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K258007_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggtatcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggcttctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggttcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcgcgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggtaacgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctgggtatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggtggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgggcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacgtgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctggcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatcagctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatccgccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcagggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctggcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatctgaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctggcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaactgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcgtatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaaggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctgcactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgctgacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaagaggagaaatactagatgactggcatggacattacgacgcaggatgaactgaacgaggctgcgatgcgtgaccgtgcctcccgtgacgaagaacgtgcactgcgtctgggttggtggctggtactggctggtttcggtggtttcctgctgtgggcgctgctggctccactggacaaaggcgtggcggtacagggcaatgtcgtggtatctggcaatcgtaaagttatccagcacatgcagggcggcatcgtggaccgcattcaagttaaggacggcgaccgcgtggctgcgggtcaagttctgctgaccctgaatgctgtggacgcacgtaccactagcgaaggtctgggttcccagtacgatcagctgatcgcacgtgaagctcgtctgctggcggagcagcgtaaccagagcagcctggcagccacgcctcgtctgactcaagctcgccagcgtccggaaatggcagcgatcattgcactgcaagaggatctgctgcgttcccgtcagcagagcctgaaactggaaattgacggcgttcgcgccagcatcgatggcctggaaaccagcctgggcgctctgcaaaaggtaatgtccagcaaacagtccgagcaagccaccctgtcccagcagctgcaaggtctgcgtccgctggctgctgacaactacgttccgcgcaacaaaatgctggaaaccgaacgcctgtttgcgcaagtatctggtgagctggctcagacttctggtgaagtgggtcgtacccgtcgtgatattcagcagcagaaactgcgtattgctcagcgtcagcaagaatacgacaaagaagtaaactccgaactgtctgacgttcaggccaaactgaacgaagtgatcagccagcgtgaaaaagccgatttcaacctggctaacgttcaggtgcgcgctccggtcgcgggcaccgtggtagacatgaaaatctttaccgagggcggtgtaatcgcgccaggtcaggtaatgatggatattgttccggaggatcagccgctgctggtagacggtcgcattcctgtagaaatggttgataaagtttggagcggtctgccagtagagctgcaattcactgcgttctctcagtctacgaccccgcgtgttccgggcaccgtcaccctgctgtctgcagaccgtctggtggatgagaaggatggtaccccgtactacggtctgcgcatccaggtttccgaggagggtaaacgcagcctgcacggcctggaaattaaacctggcatgcctgttcagggcttcgtacgtaccggtgaacgttctttcattaactatctgttcaaaccgctgatggaccgtatgcacctggctctgaccgaggaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaagaggagaaatactagatgcgtcgtaaagcggttctgctgactgtagcactgtttctgccaggtgtcggtgcgcaggcgatgggcctgctggacgcctgggaactggcgctgcgtaatgatgctcaactgcgtgctgcgggtttcgagcgcgacgcgggtcaggaggaagttgctatcggtcgcgcaggcctgctgccgtctctgcaatactcttacggcgccaactactctcactctaaagttacgcagcgcgaccgtaccaccgataacactacccagcgtgactacaacaactatgtgtctaccctgactctgcgccagccgctgctggactacgctgcttgggcccgttatcaacagggcgtaacccgtaaactgatggccgaccagcgctttcgtgaacgctcccaggacctgatggtccgcctgtaccaggcatggagcgacgcgctgctggcacaggagaaactgatgctgctggacgcacagcgccgtgcttaccaagaacagctggctctgaaccgtcgtctgctggctgccggtgaaggtacgcagaccgatctgcgtgaaactgaggcgcgctataccgttatcgaagctcagcgtatcgaacaggaagacacgctggatgcagctatgactgctctggagaatatgatcggttctcctctgcaaattcaagacctgagcccgctggtactggaagaactgcctgataatgtaaccgaaaaccgccctctgacccaatggcgtgagctggccgtacgtcacaacgcgaaactggctgtccagcgtgagaatgttgactattcccgctatgaaattgagcgcaaccgcgctggtcacctgccgtctgttgacctggtggcgtctacccgcaactccctgtctgaaagcgagtacaactacaaccagaaatatgatacccagacggtaggtgttcaggttcgtgtaccgctgtatagcggcggtgctgttagcgctagcatgcgtcaggccaccgcagaatacgcccaaaaccaggcggaactggataaccaaacgcgccagaccttcgccgaactgcgccgtcagttcaacctgtgtatcaacggtagcgcgaaaatccgcgcctggcagatgagcgtaactacggctgctgaagcgatccgtgcgacccgccagagcgttgcgggcggtgaacgcatcaacctggacgtactgatggcggagcaggagtggtataacgcccgtcgcgaactgactgaagtgaagtatcgttggctgcaagcttggctgaacctgcgttacactgccggtactctgaacgaccaggacatgatgcagctggctgcgtggttccagccggcggacgccgctaacaaaaaccgtgctaaaaccgtatctggcaataaagtgaactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K258101_sequence 1 atgactggcatggacattacgacgcaggatgaactgaacgaggctgcgatgcgtgaccgtgcctcccgtgacgaagaacgtgcactgcgtctgggttggtggctggtactggctggtttcggtggtttcctgctgtgggcgctgctggctccactggacaaaggcgtggcggtacagggcaatgtcgtggtatctggcaatcgtaaagttatccagcacatgcagggcggcatcgtggaccgcattcaagttaaggacggcgaccgcgtggctgcgggtcaagttctgctgaccctgaatgctgtggacgcacgtaccactagcgaaggtctgggttcccagtacgatcagctgatcgcacgtgaagctcgtctgctggcggagcagcgtaaccagagcagcctggcagccacgcctcgtctgactcaagctcgccagcgtccggaaatggcagcgatcattgcactgcaagaggatctgctgcgttcccgtcagcagagcctgaaactggaaattgacggcgttcgcgccagcatcgatggcctggaaaccagcctgggcgctctgcaaaaggtaatgtccagcaaacagtccgagcaagccaccctgtcccagcagctgcaaggtctgcgtccgctggctgctgacaactacgttccgcgcaacaaaatgctggaaaccgaacgcctgtttgcgcaagtatctggtgagctggctcagacttctggtgaagtgggtcgtacccgtcgtgatattcagcagcagaaactgcgtattgctcagcgtcagcaagaatacgacaaagaagtaaactccgaactgtctgacgttcaggccaaactgaacgaagtgatcagccagcgtgaaaaagccgatttcaacctggctaacgttcaggtgcgcgctccggtcgcgggcaccgtggtagacatgaaaatctttaccgagggcggtgtaatcgcgccaggtcaggtaatgatggatattgttccggaggatcagccgctgctggtagacggtcgcattcctgtagaaatggttgataaagtttggagcggtctgccagtagagctgcaattcactgcgttctctcagtctacgaccccgcgtgttccgggcaccgtcaccctgctgtctgcagaccgtctggtggatgagaaggatggtaccccgtactacggtctgcgcatccaggtttccgaggagggtaaacgcagcctgcacggcctggaaattaaacctggcatgcctgttcagggcttcgtacgtaccggtgaacgttctttcattaactatctgttcaaaccgctgatggaccgtatgcacctggctctgaccgaggaa BBa_K258100_sequence 1 atgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggtatcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggcttctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggttcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcgcgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggtaacgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctgggtatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggtggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgggcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacgtgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctggcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatcagctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatccgccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcagggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctggcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatctgaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctggcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaactgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcgtatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaaggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctgcactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgctgacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcag BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K258102_sequence 1 atgcgtcgtaaagcggttctgctgactgtagcactgtttctgccaggtgtcggtgcgcaggcgatgggcctgctggacgcctgggaactggcgctgcgtaatgatgctcaactgcgtgctgcgggtttcgagcgcgacgcgggtcaggaggaagttgctatcggtcgcgcaggcctgctgccgtctctgcaatactcttacggcgccaactactctcactctaaagttacgcagcgcgaccgtaccaccgataacactacccagcgtgactacaacaactatgtgtctaccctgactctgcgccagccgctgctggactacgctgcttgggcccgttatcaacagggcgtaacccgtaaactgatggccgaccagcgctttcgtgaacgctcccaggacctgatggtccgcctgtaccaggcatggagcgacgcgctgctggcacaggagaaactgatgctgctggacgcacagcgccgtgcttaccaagaacagctggctctgaaccgtcgtctgctggctgccggtgaaggtacgcagaccgatctgcgtgaaactgaggcgcgctataccgttatcgaagctcagcgtatcgaacaggaagacacgctggatgcagctatgactgctctggagaatatgatcggttctcctctgcaaattcaagacctgagcccgctggtactggaagaactgcctgataatgtaaccgaaaaccgccctctgacccaatggcgtgagctggccgtacgtcacaacgcgaaactggctgtccagcgtgagaatgttgactattcccgctatgaaattgagcgcaaccgcgctggtcacctgccgtctgttgacctggtggcgtctacccgcaactccctgtctgaaagcgagtacaactacaaccagaaatatgatacccagacggtaggtgttcaggttcgtgtaccgctgtatagcggcggtgctgttagcgctagcatgcgtcaggccaccgcagaatacgcccaaaaccaggcggaactggataaccaaacgcgccagaccttcgccgaactgcgccgtcagttcaacctgtgtatcaacggtagcgcgaaaatccgcgcctggcagatgagcgtaactacggctgctgaagcgatccgtgcgacccgccagagcgttgcgggcggtgaacgcatcaacctggacgtactgatggcggagcaggagtggtataacgcccgtcgcgaactgactgaagtgaagtatcgttggctgcaagcttggctgaacctgcgttacactgccggtactctgaacgaccaggacatgatgcagctggctgcgtggttccagccggcggacgccgctaacaaaaaccgtgctaaaaccgtatctggcaataaagtgaac BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z