Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
Cihan Tastan donated by Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
Sequence for pTet inverting regulator driven by the TetR protein.
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
PrtD domain of ABC transporter of Erwinia chrysanthemi
E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 60 kd protein to the prtD gene product (protein PrtD).
The predicted amino acid sequence of the PrtD protein indicates that this protein has a putative ATP binding site, located in the C-terminal half of the protein, which corresponds to the consensus sequence proposed for ATP binding proteins.
The gene actually will be fully function with other two genes which are PrtD and PrtE
PrtE domain of ABC transporter of Erwinia chrysanthemi
E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 50 kd protein to the prtD gene product (protein PrtD).
prtE, codes for a polypeptide of 448 amino acid residues (predicted Mr = 49.5 kd) which is probably initiated at an ATG codon located 14 bp downstream from the prtD gene stop codon TGA. It is preceded 11 bp upstream by a GGGA RBS consensus sequence.The hydropathy profile of the PrtE protein shows at least one hydrophobic domain with the sequence (residues 30 to 60) which might be a transmembrane segment. No signal peptide is found at the N-terminus, which is hydrophilic
The gene actually will be fully function with other two genes which are PrtD and PrtF.
PrtF domain of ABC transporter of Erwinia chrysanthemi
E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.
prtF, codes for a polypeptide of 462 amino acid residues (predicted Mr = 52.5 kd) that is probably initiated at an ATG codon located 15 bp downstream from the prtE gene stop codon TAA. It is preceded 11 bp upstream by a GGAAGG RBS consensus sequence.the PrtF protein is hydrophilic and analysis of the deduced amino acid sequence revealed the presence at its N-terminal end of a typical procaryotic signal peptide (italics in Figure 2) which contains three positively charged residues followed by a stretch of hydrophobic or neutral residues and ending at a consensus bacterial signal peptide cleavage site, Ala Gln Ala or Ala Met Gly.
The gene actually will be fully function with other two genes which are PrtD and PrtE.
Conversion of the iGEM parts registry to SBOL2.1