PrtDEF
type: ComponentDefinition
persistent identity: BBa K258007
display id: BBa_K258007
version: 1
was generated by: iGEM to SBOL conversion
description: Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
owned by: james
owned by: myers
discontinued: false
dominant: true
experience: experience/Works
group u list: _9_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Cihan Tastan donated by Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_R0040
component: BBa_B0034
component: BBa_B0010
component: BBa_K258100
component: BBa_K258102
component: BBa_K258101
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K258100
sequence annotation: BBa_B0010
sequence annotation: BBa_K258101
sequence annotation: BBa_K258102
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K258007_sequence/1

BBa_R0040
type: Component
persistent identity: component2033363
display id: component2033363
version: 1
top level: PrtDEF
definition: p(tetR)
access: public

BBa_B0034
type: Component
persistent identity: component2033374
display id: component2033374
version: 1
top level: PrtDEF
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2033371
display id: component2033371
version: 1
top level: PrtDEF
definition: BBa_B0010
access: public

BBa_K258100
type: Component
persistent identity: component2033370
display id: component2033370
version: 1
top level: PrtDEF
definition: BBa_K258100
access: public

BBa_B0034
type: Component
persistent identity: component2033369
display id: component2033369
version: 1
top level: PrtDEF
definition: BBa_B0034
access: public

BBa_K258102
type: Component
persistent identity: component2033380
display id: component2033380
version: 1
top level: PrtDEF
definition: BBa_K258102
access: public

BBa_B0034
type: Component
persistent identity: component2033379
display id: component2033379
version: 1
top level: PrtDEF
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2033376
display id: component2033376
version: 1
top level: PrtDEF
definition: BBa_B0010
access: public

BBa_K258101
type: Component
persistent identity: component2033375
display id: component2033375
version: 1
top level: PrtDEF
definition: BBa_K258101
access: public

BBa_B0012
type: Component
persistent identity: component2033383
display id: component2033383
version: 1
top level: PrtDEF
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2033381
display id: component2033381
version: 1
top level: PrtDEF
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2033363
display id: annotation2033363
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033363/range2033363/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K258007/annotation2033363/range2033363/1
type: Range
persistent identity: range2033363
display id: range2033363
version: 1
top level: PrtDEF
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033369
display id: annotation2033369
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033369/range2033369/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258007/annotation2033369/range2033369/1
type: Range
persistent identity: range2033369
display id: range2033369
version: 1
top level: PrtDEF
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K258100
type: SequenceAnnotation
persistent identity: annotation2033370
display id: annotation2033370
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033370/range2033370/1
component: BBa_K258100

https://synbiohub.org/public/igem/BBa_K258007/annotation2033370/range2033370/1
type: Range
persistent identity: range2033370
display id: range2033370
version: 1
top level: PrtDEF
direction: direction/reverse
start: 81
end: 1805
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033371
display id: annotation2033371
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033371/range2033371/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258007/annotation2033371/range2033371/1
type: Range
persistent identity: range2033371
display id: range2033371
version: 1
top level: PrtDEF
direction: direction/forward
start: 1814
end: 1893
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033374
display id: annotation2033374
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033374/range2033374/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258007/annotation2033374/range2033374/1
type: Range
persistent identity: range2033374
display id: range2033374
version: 1
top level: PrtDEF
direction: direction/reverse
start: 1902
end: 1913
orientation: inline

BBa_K258101
type: SequenceAnnotation
persistent identity: annotation2033375
display id: annotation2033375
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033375/range2033375/1
component: BBa_K258101

https://synbiohub.org/public/igem/BBa_K258007/annotation2033375/range2033375/1
type: Range
persistent identity: range2033375
display id: range2033375
version: 1
top level: PrtDEF
direction: direction/reverse
start: 1920
end: 3263
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033376
display id: annotation2033376
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033376/range2033376/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258007/annotation2033376/range2033376/1
type: Range
persistent identity: range2033376
display id: range2033376
version: 1
top level: PrtDEF
direction: direction/forward
start: 3272
end: 3351
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033379
display id: annotation2033379
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033379/range2033379/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258007/annotation2033379/range2033379/1
type: Range
persistent identity: range2033379
display id: range2033379
version: 1
top level: PrtDEF
direction: direction/reverse
start: 3360
end: 3371
orientation: inline

BBa_K258102
type: SequenceAnnotation
persistent identity: annotation2033380
display id: annotation2033380
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033380/range2033380/1
component: BBa_K258102

https://synbiohub.org/public/igem/BBa_K258007/annotation2033380/range2033380/1
type: Range
persistent identity: range2033380
display id: range2033380
version: 1
top level: PrtDEF
direction: direction/reverse
start: 3378
end: 4763
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033381
display id: annotation2033381
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033381/range2033381/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258007/annotation2033381/range2033381/1
type: Range
persistent identity: range2033381
display id: range2033381
version: 1
top level: PrtDEF
direction: direction/forward
start: 4772
end: 4851
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2033383
display id: annotation2033383
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258007/annotation2033383/range2033383/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K258007/annotation2033383/range2033383/1
type: Range
persistent identity: range2033383
display id: range2033383
version: 1
top level: PrtDEF
direction: direction/forward
start: 4860
end: 4900
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K258100
type: ComponentDefinition
persistent identity: BBa K258100
display id: BBa_K258100
version: 1
was generated by: iGEM to SBOL conversion
description: PrtD domain of ABC transporter of Erwinia chrysanthemi
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 60 kd protein to the prtD gene product (protein PrtD).
owned by: james
owned by: myers
mutable description: The predicted amino acid sequence of the PrtD protein indicates that this protein has a putative ATP binding site, located in the C-terminal half of the protein, which corresponds to the consensus sequence proposed for ATP binding proteins.
discontinued: false
dominant: false
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The gene actually will be fully function with other two genes which are PrtD and PrtE
star: false
creator: Cihan Tastan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K258100_sequence/1

BBa_K258101
type: ComponentDefinition
persistent identity: BBa K258101
display id: BBa_K258101
version: 1
was generated by: iGEM to SBOL conversion
description: PrtE domain of ABC transporter of Erwinia chrysanthemi
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 50 kd protein to the prtD gene product (protein PrtD).
owned by: james
owned by: myers
mutable description: prtE, codes for a polypeptide of 448 amino acid residues (predicted Mr = 49.5 kd) which is probably initiated at an ATG codon located 14 bp downstream from the prtD gene stop codon TGA. It is preceded 11 bp upstream by a GGGA RBS consensus sequence.The hydropathy profile of the PrtE protein shows at least one hydrophobic domain with the sequence (residues 30 to 60) which might be a transmembrane segment. No signal peptide is found at the N-terminus, which is hydrophilic
discontinued: false
dominant: false
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The gene actually will be fully function with other two genes which are PrtD and PrtF.
star: false
creator: Cihan Tastan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K258101_sequence/1

BBa_K258102
type: ComponentDefinition
persistent identity: BBa K258102
display id: BBa_K258102
version: 1
was generated by: iGEM to SBOL conversion
description: PrtF domain of ABC transporter of Erwinia chrysanthemi
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.
owned by: james
owned by: myers
mutable description: prtF, codes for a polypeptide of 462 amino acid residues (predicted Mr = 52.5 kd) that is probably initiated at an ATG codon located 15 bp downstream from the prtE gene stop codon TAA. It is preceded 11 bp upstream by a GGAAGG RBS consensus sequence.the PrtF protein is hydrophilic and analysis of the deduced amino acid sequence revealed the presence at its N-terminal end of a typical procaryotic signal peptide (italics in Figure 2) which contains three positively charged residues followed by a stretch of hydrophobic or neutral residues and ending at a consensus bacterial signal peptide cleavage site, Ala Gln Ala or Ala Met Gly.
discontinued: false
dominant: false
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The gene actually will be fully function with other two genes which are PrtD and PrtE.
star: false
creator: Cihan Tastan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K258102_sequence/1

https://synbiohub.org/public/igem/BBa_K258007_sequence/1
type: Sequence
persistent identity: BBa K258007 sequence
display id: BBa_K258007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggtatcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggcttctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggttcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcgcgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggtaacgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctgggtatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggtggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgggcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacgtgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctggcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatcagctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatccgccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcagggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctggcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatctgaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctggcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaactgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcgtatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaaggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctgcactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgctgacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaagaggagaaatactagatgactggcatggacattacgacgcaggatgaactgaacgaggctgcgatgcgtgaccgtgcctcccgtgacgaagaacgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258100_sequence/1
type: Sequence
persistent identity: BBa K258100 sequence
display id: BBa_K258100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggtatcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggcttctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggttcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcgcgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggtaacgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctgggtatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggtggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgggcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacgtgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctggcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatcagctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatccgccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcagggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctggcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatctgaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctggcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaactgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcgtatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaaggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctgcactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgctgacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258101_sequence/1
type: Sequence
persistent identity: BBa K258101 sequence
display id: BBa_K258101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactggcatggacattacgacgcaggatgaactgaacgaggctgcgatgcgtgaccgtgcctcccgtgacgaagaacgtgcactgcgtctgggttggtggctggtactggctggtttcggtggtttcctgctgtgggcgctgctggctccactggacaaaggcgtggcggtacagggcaatgtcgtggtatctggcaatcgtaaagttatccagcacatgcagggcggcatcgtggaccgcattcaagttaaggacggcgaccgcgtggctgcgggtcaagttctgctgaccctgaatgctgtggacgcacgtaccactagcgaaggtctgggttcccagtacgatcagctgatcgcacgtgaagctcgtctgctggcggagcagcgtaaccagagcagcctggcagccacgcctcgtctgactcaagctcgccagcgtccggaaatggcagcgatcattgcactgcaagaggatctgctgcgttcccgtcagcagagcctgaaactggaaattgacggcgttcgcgccagcatcgatggcctggaaaccagcctgggcgctctgcaaaaggtaatgtccagcaaacagtccgagcaagccaccctgtcccagcagctgcaaggtctgcgtccgctggctgctgacaactacgttccgcgcaacaaaatgctggaaaccgaacgcctgtttgcgcaagtatctggtgagctggctcagacttctggtgaagtgggtcgtacccgtcgtgatattcagcagcagaaactgcgtattgctcagcgtcagcaagaatacgacaaagaagtaaactccgaactgtctgacgttcaggccaaactgaacgaagtgatcagccagcgtgaaaaagccgatttcaacctggctaacgttcaggtgcgcgctccggtcgcgggcaccgtggtagacatgaaaatctttaccgagggcggtgtaatcgcgccaggtcaggtaatgatggatattgttccggaggatcagccgctgctggtagacggtcgcattcctgtagaaatggttgataaagtttggagcggtctgccagtagagctgcaattcactgcgttctctcagtctacgaccccgcgtgttccgggcaccgtcaccctgctgtctgcagaccgtctggtggatgagaaggatggtaccccgtactacggtctgcgcatccaggtttccgaggagggtaaacgcagcctgcacggcctggaaattaaacctggcatgcctgttcagggcttcgtacgtaccggtgaacgttctttcattaactatctgttcaaaccgctgatggaccgtatgcacctggctctgaccgaggaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258102_sequence/1
type: Sequence
persistent identity: BBa K258102 sequence
display id: BBa_K258102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtcgtaaagcggttctgctgactgtagcactgtttctgccaggtgtcggtgcgcaggcgatgggcctgctggacgcctgggaactggcgctgcgtaatgatgctcaactgcgtgctgcgggtttcgagcgcgacgcgggtcaggaggaagttgctatcggtcgcgcaggcctgctgccgtctctgcaatactcttacggcgccaactactctcactctaaagttacgcagcgcgaccgtaccaccgataacactacccagcgtgactacaacaactatgtgtctaccctgactctgcgccagccgctgctggactacgctgcttgggcccgttatcaacagggcgtaacccgtaaactgatggccgaccagcgctttcgtgaacgctcccaggacctgatggtccgcctgtaccaggcatggagcgacgcgctgctggcacaggagaaactgatgctgctggacgcacagcgccgtgcttaccaagaacagctggctctgaaccgtcgtctgctggctgccggtgaaggtacgcagaccgatctgcgtgaaactgaggcgcgctataccgttatcgaagctcagcgtatcgaacaggaagacacgctggatgcagctatgactgctctggagaatatgatcggttctcctctgcaaattcaagacctgagcccgctggtactggaagaactgcctgataatgtaaccgaaaaccgccctctgacccaatggcgtgagctggccgtacgtcacaacgcgaaactggctgtccagcgtgagaatgttgactattcccgctatgaaattgagcgcaaccgcgctggtcacctgccgtctgttgacctggtggcgtctacccgcaactccctgtctgaaagcgagtacaactacaaccagaaatatgatacccagacggtaggtgttcaggttcgtgtaccgctgtatagcggcggtgctgttagcgctagcatgcgtcaggccaccgcagaatacgcccaaaaccaggcggaactggataaccaaacgcgccagaccttcgccgaactgcgccgtcagttcaacctgtgtatcaacggtagcgcgaaaatccgcgcctggcagatgagcgtaactacggctgctgaagcgatccgtgcgacccgccagagcgttgcgggcggtgaacgcatcaacctggacgtactgatggcggagcaggagtggtataacgcccgtcgcgaactgactgaagtgaagtatcgttggctgcaagcttggctgaacctgcgttacactgccggtactctgaacgaccaggacatgatgcagctggctgcgtggttccagccggcggacgccgctaacaaaaaccgtgctaaaaccgtatctggcaataaagtgaac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z