##gff-version 3
##sequence-region BBa_K258008 1 4900
BBa_K258008	.	terminator	4772	4851	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K258008	.	stem_loop	4783	4826	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K258008	.	engineered_region	4772	4851	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K258008	.	CDS	1920	3263	.	+	0	ID=BBa_K258101;Name=BBa_K258101
BBa_K258008	.	ribosome_entry_site	1902	1913	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K258008	.	conserved_region	1906	1909	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K258008	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K258008	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K258008	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K258008	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K258008	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K258008	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K258008	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K258008	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K258008	.	terminator	1814	1893	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K258008	.	stem_loop	1825	1868	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K258008	.	engineered_region	1814	1893	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K258008	.	CDS	81	1805	.	+	0	ID=BBa_K258100;Name=BBa_K258100
BBa_K258008	.	terminator	4860	4900	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K258008	.	polyA_site	4887	4900	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K258008	.	stem_loop	4867	4886	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K258008	.	engineered_region	4860	4900	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K258008	.	stop_codon	4893	4893	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K258008	.	terminator	3272	3351	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K258008	.	stem_loop	3283	3326	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K258008	.	engineered_region	3272	3351	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K258008	.	CDS	3378	4763	.	+	0	ID=BBa_K258102;Name=BBa_K258102
BBa_K258008	.	ribosome_entry_site	3360	3371	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K258008	.	conserved_region	3364	3367	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K258008
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggt
atcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggctt
ctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggt
tcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcg
cgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggta
acgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctggg
tatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggt
ggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgg
gcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacg
tgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctg
gcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatc
agctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatcc
gccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcag
ggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctg
gcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatct
gaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctg
gcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaac
tgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcg
tatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaa
ggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctg
cactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgct
gacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcagtactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagaaagaggagaaatactagatgactggcatggacattacgacgcaggatgaactgaacgaggctgcgatgcgtga
ccgtgcctcccgtgacgaagaacgtgcactgcgtctgggttggtggctggtactggctggtttcggtggtttcctgctg
tgggcgctgctggctccactggacaaaggcgtggcggtacagggcaatgtcgtggtatctggcaatcgtaaagttatcc
agcacatgcagggcggcatcgtggaccgcattcaagttaaggacggcgaccgcgtggctgcgggtcaagttctgctgac
cctgaatgctgtggacgcacgtaccactagcgaaggtctgggttcccagtacgatcagctgatcgcacgtgaagctcgt
ctgctggcggagcagcgtaaccagagcagcctggcagccacgcctcgtctgactcaagctcgccagcgtccggaaatgg
cagcgatcattgcactgcaagaggatctgctgcgttcccgtcagcagagcctgaaactggaaattgacggcgttcgcgc
cagcatcgatggcctggaaaccagcctgggcgctctgcaaaaggtaatgtccagcaaacagtccgagcaagccaccctg
tcccagcagctgcaaggtctgcgtccgctggctgctgacaactacgttccgcgcaacaaaatgctggaaaccgaacgcc
tgtttgcgcaagtatctggtgagctggctcagacttctggtgaagtgggtcgtacccgtcgtgatattcagcagcagaa
actgcgtattgctcagcgtcagcaagaatacgacaaagaagtaaactccgaactgtctgacgttcaggccaaactgaac
gaagtgatcagccagcgtgaaaaagccgatttcaacctggctaacgttcaggtgcgcgctccggtcgcgggcaccgtgg
tagacatgaaaatctttaccgagggcggtgtaatcgcgccaggtcaggtaatgatggatattgttccggaggatcagcc
gctgctggtagacggtcgcattcctgtagaaatggttgataaagtttggagcggtctgccagtagagctgcaattcact
gcgttctctcagtctacgaccccgcgtgttccgggcaccgtcaccctgctgtctgcagaccgtctggtggatgagaagg
atggtaccccgtactacggtctgcgcatccaggtttccgaggagggtaaacgcagcctgcacggcctggaaattaaacc
tggcatgcctgttcagggcttcgtacgtaccggtgaacgttctttcattaactatctgttcaaaccgctgatggaccgt
atgcacctggctctgaccgaggaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagaaagaggagaaatactagatgcgtcgtaaagcggttct
gctgactgtagcactgtttctgccaggtgtcggtgcgcaggcgatgggcctgctggacgcctgggaactggcgctgcgt
aatgatgctcaactgcgtgctgcgggtttcgagcgcgacgcgggtcaggaggaagttgctatcggtcgcgcaggcctgc
tgccgtctctgcaatactcttacggcgccaactactctcactctaaagttacgcagcgcgaccgtaccaccgataacac
tacccagcgtgactacaacaactatgtgtctaccctgactctgcgccagccgctgctggactacgctgcttgggcccgt
tatcaacagggcgtaacccgtaaactgatggccgaccagcgctttcgtgaacgctcccaggacctgatggtccgcctgt
accaggcatggagcgacgcgctgctggcacaggagaaactgatgctgctggacgcacagcgccgtgcttaccaagaaca
gctggctctgaaccgtcgtctgctggctgccggtgaaggtacgcagaccgatctgcgtgaaactgaggcgcgctatacc
gttatcgaagctcagcgtatcgaacaggaagacacgctggatgcagctatgactgctctggagaatatgatcggttctc
ctctgcaaattcaagacctgagcccgctggtactggaagaactgcctgataatgtaaccgaaaaccgccctctgaccca
atggcgtgagctggccgtacgtcacaacgcgaaactggctgtccagcgtgagaatgttgactattcccgctatgaaatt
gagcgcaaccgcgctggtcacctgccgtctgttgacctggtggcgtctacccgcaactccctgtctgaaagcgagtaca
actacaaccagaaatatgatacccagacggtaggtgttcaggttcgtgtaccgctgtatagcggcggtgctgttagcgc
tagcatgcgtcaggccaccgcagaatacgcccaaaaccaggcggaactggataaccaaacgcgccagaccttcgccgaa
ctgcgccgtcagttcaacctgtgtatcaacggtagcgcgaaaatccgcgcctggcagatgagcgtaactacggctgctg
aagcgatccgtgcgacccgccagagcgttgcgggcggtgaacgcatcaacctggacgtactgatggcggagcaggagtg
gtataacgcccgtcgcgaactgactgaagtgaagtatcgttggctgcaagcttggctgaacctgcgttacactgccggt
actctgaacgaccaggacatgatgcagctggctgcgtggttccagccggcggacgccgctaacaaaaaccgtgctaaaa
ccgtatctggcaataaagtgaactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta