LOCUS BBa_K258011 3552 bp DNA linear 21-May-2024 DEFINITION Oxygen-dependent EGF synthesizing with GFP reporter ACCESSION BBa_K258011 VERSION BBa_K258011.1 FEATURES Location/Qualifiers promoter 1..137 /label=BBa_K258005 misc_feature 150..153 /label=conserved RBS 146..157 /label=BBa_B0034 CDS 167..783 /label=tetR misc_feature 784..817 /label=SsrA misc_feature 824..848 /label=Help:Barcodes CDS 164..823 /label=BBa_C0040 stem_loop 868..911 /label=stem_loop misc_feature 857..936 /label=BBa_B0010 terminator 857..936 /label=BBa_B0010 stem_loop 952..971 /label=T7 TE misc_feature 978..978 /label=stop polyA_site 972..985 /label=polya misc_feature 945..985 /label=BBa_B0012 terminator 945..985 /label=BBa_B0012 misc_feature 994..1012 /label=TetR 1 misc_feature 994..1047 /label=BBa_R0040 promoter 1013..1018 /label=-35 misc_feature 1019..1037 /label=TetR 2 promoter 1036..1041 /label=-10 promoter 994..1047 /label=BBa_R0040 misc_feature 146..1047 /label=BBa_Q04400 misc_feature 1060..1063 /label=conserved RBS 1056..1067 /label=BBa_B0034 CDS 1074..1235 /label=BBa_K258010 misc_feature 1242..2669 /label=BBa_K258006 misc_feature 2682..2685 /label=conserved RBS 2678..2689 /label=BBa_B0034 CDS 2696..3415 /label=GFP protein CDS 2696..3415 /label=BBa_E0040 misc_feature 2678..3415 /label=BBa_I13500 stem_loop 3435..3478 /label=stem_loop misc_feature 3424..3503 /label=BBa_B0010 terminator 3424..3503 /label=BBa_B0010 stem_loop 3519..3538 /label=T7 TE misc_feature 3545..3545 /label=stop polyA_site 3539..3552 /label=polya misc_feature 3512..3552 /label=BBa_B0012 terminator 3512..3552 /label=BBa_B0012 terminator 3424..3552 /label=BBa_B0015 ORIGIN 1 aagcttacag gacgctgggg ttaaagtatt tgagttttga tgtggattaa gtttagaggc 61 aataaagatt ataataagtg ctgctacacc atactgatgt atggcaaacc ataataatga 121 acttaaggaa gaccctctac tagagaaaga ggagaaatac tagatgtcca gattagataa 181 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac 241 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa 301 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca 361 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt 421 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac 481 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc 541 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt 601 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat 661 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt 721 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg 781 gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg 841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 961 tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga gattgacatc 1021 cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac tagatgaaca 1081 gcgactcgga gtgtccactg tcacatgatg gctattgcct gcacgatggc gtttgtatgt 1141 atatcgaggc cctggacaaa tatgcctgta actgtgtggt gggctatatc ggtgaacgtt 1201 gccagtatcg tgacctgaaa tggtgggaac tgcgttacta gatgggtgtg ttcgattaca 1261 agaacctggg tacggaagcc tctaaaaccc tgttcgctga tgcgaccgcg atcaccctgt 1321 atacctacca caacctggat aacggtttcg ctgtaggtta tcagcagcac ggcctgggtc 1381 tgggcctgcc agcgaccctg gtcggcgcgc tgctgggttc taccgactct caaggcgtga 1441 tcccgggcat cccatggaac ccggactctg aaaaagcagc tctggacgcg gtacatgcag 1501 ctggctggac tccgatctcc gcttctgcgc tgggctacgg tggcaaagtc gacgctcgtg 1561 gcaccttctt cggcgaaaaa gcaggctata ccacggcaca ggccgaagta ctgggcaagt 1621 acgacgatgc tggtaaactg ctggaaatcg gcatcggttt ccgtggcacc agcggtccgc 1681 gtgaatccct gatcactgac tccatcggtg atctggtaag cgatctgctg gcggctctgg 1741 gtccgaagga ctacgcgaaa aactacgcag gtgaagcctt cggtggtctg ctgaagactg 1801 ttgcagacta cgccggtgcc cacggtctgt ccggtaagga cgtcctggtg tccggtcata 1861 gcctgggtgg tctggcggtg aactctatgg cagatctgag caccagcaag tgggcgggct 1921 tttacaaaga tgcaaactac ctggcttacg cctctccaac gcagtctgct ggtgataaag 1981 tcctgaacat cggttacgaa aacgacccgg tctttcgtgc tctggacggt tccactttca 2041 acctgtcctc tctgggtgta cacgacaaag cccacgaatc cactacggac aacattgtca 2101 gctttaacga ccactatgcg tctactctgt ggaacgtcct gccgttctct attgccaacc 2161 tgtccacttg ggtgtctcac ctgccgtctg cttacggtga cggcatgacc cgtgttctgg 2221 aaagcggctt ctacgaacag atgacccgtg attccactat tattgtggct aacctgtccg 2281 acccggcgcg cgctaatacg tgggttcagg atctgaaccg taacgcagaa ccgcataccg 2341 gcaatacttt catcatcggt tctgatggta atgacctgat tcagggcggt aaaggtgctg 2401 atttcatcga gggcggcaaa ggtaatgaca ctatccgtga taattccggc cacaatacgt 2461 tcctgttttc cggtcacttc ggccaggacc gtattatcgg ctatcagccg accgaccgtc 2521 tggtgttcca gggtgcggac ggtagcaccg acctgcgtga ccacgcgaaa gccgtgggcg 2581 ctgacaccgt tctgtctttc ggcgcagact ctgtgactct ggtcggcgtc ggcctgggtg 2641 gcctgtggtc tgaaggcgtt ctgatttctt actagagaaa gaggagaaat actagatgcg 2701 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt 2761 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact 2821 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac 2881 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga 2941 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga 3001 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag 3061 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga 3121 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa 3181 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta 3241 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc 3301 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga 3361 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta 3421 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 3481 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 3541 tctgcgttta ta //