##gff-version 3
##sequence-region BBa_K258011 1 3552
BBa_K258011	.	engineered_region	146	1047	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_K258011	.	terminator	857	936	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K258011	.	engineered_region	857	936	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K258011	.	stem_loop	868	911	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K258011	.	terminator	945	985	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K258011	.	stem_loop	952	971	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K258011	.	engineered_region	945	985	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K258011	.	polyA_site	972	985	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K258011	.	stop_codon	978	978	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K258011	.	promoter	994	1047	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K258011	.	promoter	1036	1041	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K258011	.	promoter	1013	1018	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K258011	.	engineered_region	994	1047	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K258011	.	non_covalent_binding_site	1019	1037	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K258011	.	non_covalent_binding_site	994	1012	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K258011	.	ribosome_entry_site	146	157	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K258011	.	conserved_region	150	153	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K258011	.	CDS	164	823	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K258011	.	engineered_tag	824	848	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K258011	.	CDS	167	783	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K258011	.	tag	784	817	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K258011	.	terminator	3424	3552	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K258011	.	terminator	3424	3503	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K258011	.	engineered_region	3424	3503	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K258011	.	stem_loop	3435	3478	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K258011	.	terminator	3512	3552	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K258011	.	stem_loop	3519	3538	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K258011	.	engineered_region	3512	3552	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K258011	.	polyA_site	3539	3552	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K258011	.	stop_codon	3545	3545	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K258011	.	promoter	1	137	.	+	0	ID=BBa_K258005;Name=BBa_K258005
BBa_K258011	.	engineered_region	2678	3415	.	+	0	ID=BBa_I13500;Name=BBa_I13500
BBa_K258011	.	ribosome_entry_site	2678	2689	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13500
BBa_K258011	.	conserved_region	2682	2685	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K258011	.	CDS	2696	3415	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13500
BBa_K258011	.	CDS	2696	3415	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K258011	.	ribosome_entry_site	1056	1067	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K258011	.	conserved_region	1060	1063	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K258011	.	CDS	1074	1235	.	+	0	ID=BBa_K258010;Name=BBa_K258010
BBa_K258011	.	tag	1242	2669	.	+	0	ID=BBa_K258006;Name=BBa_K258006
>BBa_K258011
aagcttacaggacgctggggttaaagtatttgagttttgatgtggattaagtttagaggcaataaagattataataagt
gctgctacaccatactgatgtatggcaaaccataataatgaacttaaggaagaccctctactagagaaagaggagaaat
actagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttt
aacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttg
ctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatt
ttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacg
gcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgca
ttatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaag
aaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcaga
gccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgca
aacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatca
gtgatagagatactgagcactactagagaaagaggagaaatactagatgaacagcgactcggagtgtccactgtcacat
gatggctattgcctgcacgatggcgtttgtatgtatatcgaggccctggacaaatatgcctgtaactgtgtggtgggct
atatcggtgaacgttgccagtatcgtgacctgaaatggtgggaactgcgttactagatgggtgtgttcgattacaagaa
cctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataac
ggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttcta
ccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagc
tggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaa
gcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtt
tccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctct
gggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggt
gcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatc
tgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtga
taaagtcctgaacatcggttacgaaaacgacccggtctttcgtgctctggacggttccactttcaacctgtcctctctg
ggtgtacacgacaaagcccacgaatccactacggacaacattgtcagctttaacgaccactatgcgtctactctgtgga
acgtcctgccgttctctattgccaacctgtccacttgggtgtctcacctgccgtctgcttacggtgacggcatgacccg
tgttctggaaagcggcttctacgaacagatgacccgtgattccactattattgtggctaacctgtccgacccggcgcgc
gctaatacgtgggttcaggatctgaaccgtaacgcagaaccgcataccggcaatactttcatcatcggttctgatggta
atgacctgattcagggcggtaaaggtgctgatttcatcgagggcggcaaaggtaatgacactatccgtgataattccgg
ccacaatacgttcctgttttccggtcacttcggccaggaccgtattatcggctatcagccgaccgaccgtctggtgttc
cagggtgcggacggtagcaccgacctgcgtgaccacgcgaaagccgtgggcgctgacaccgttctgtctttcggcgcag
actctgtgactctggtcggcgtcggcctgggtggcctgtggtctgaaggcgttctgatttcttactagagaaagaggag
aaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatg
ggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactac
tggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagat
catatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatg
acgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattga
ttttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagac
aaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatt
atcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttc
gaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggat
gaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata