BBa_K258011
type: ComponentDefinition
persistent identity: BBa K258011
display id: BBa_K258011
version: 1
was generated by: iGEM to SBOL conversion
description: Oxygen-dependent EGF synthesizing with GFP reporter
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: EGF will be activated with oxygen presence using tetR inverter mechanism. Therefore,with the absence of oxygen tetR will be synthesized so that EGF and GFP synthesize will be stopped.
owned by: james
owned by: myers
mutable description: the Epidermal Growth Factor(EGF) has a big role in wound healing.Moreover, it has also a function to start the formation of keratin and to stimulate cell division. It can also increase the amount of nucleic acids.And, it causes the storage of glikozamin and glikozaminoglikan.In structural perspective, it is composed of 53 aminoacids and has three disulfide bond.Although these positive effect of EGF, with the abundance, it can show ,for example, a trigger effect of tumor cells formation. Another side effect is to start the formation of vein.As a result, it may show a trigger for cancer unless unregulated and too much synthesize..
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: design with biobrick assembly
star: false
creator: Cihan Tastan and Ozkan IS
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_Q04400
component: BBa_K258005
component: BBa_B0015
component: BBa_I13500
component: BBa_K258006
component: BBa_K258010
sequence annotation: BBa_K258005
sequence annotation: BBa_Q04400
sequence annotation: BBa_B0034
sequence annotation: BBa_K258010
sequence annotation: BBa_K258006
sequence annotation: BBa_I13500
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K258011_sequence/1

BBa_B0034
type: Component
persistent identity: component2300232
display id: component2300232
version: 1
top level: BBa_K258011
definition: BBa_B0034
access: public

BBa_Q04400
type: Component
persistent identity: component2300230
display id: component2300230
version: 1
top level: BBa_K258011
definition: tetR
access: public

BBa_K258005
type: Component
persistent identity: component2300212
display id: component2300212
version: 1
top level: BBa_K258011
definition: BBa_K258005
access: public

BBa_B0015
type: Component
persistent identity: component2300245
display id: component2300245
version: 1
top level: BBa_K258011
definition: BBa_B0015
access: public

BBa_I13500
type: Component
persistent identity: component2300238
display id: component2300238
version: 1
top level: BBa_K258011
definition: BBa_I13500
access: public

BBa_K258006
type: Component
persistent identity: component2300234
display id: component2300234
version: 1
top level: BBa_K258011
definition: BBa_K258006
access: public

BBa_K258010
type: Component
persistent identity: component2300233
display id: component2300233
version: 1
top level: BBa_K258011
definition: BBa_K258010
access: public

BBa_K258005
type: SequenceAnnotation
persistent identity: annotation2300212
display id: annotation2300212
version: 1
top level: BBa_K258011
location: https://synbiohub.org/public/igem/BBa_K258011/annotation2300212/range2300212/1
component: BBa_K258005

https://synbiohub.org/public/igem/BBa_K258011/annotation2300212/range2300212/1
type: Range
persistent identity: range2300212
display id: range2300212
version: 1
top level: BBa_K258011
direction: direction/reverse
start: 1
end: 137
orientation: inline

BBa_Q04400
type: SequenceAnnotation
persistent identity: annotation2300230
display id: annotation2300230
version: 1
top level: BBa_K258011
location: https://synbiohub.org/public/igem/BBa_K258011/annotation2300230/range2300230/1
component: BBa_Q04400

https://synbiohub.org/public/igem/BBa_K258011/annotation2300230/range2300230/1
type: Range
persistent identity: range2300230
display id: range2300230
version: 1
top level: BBa_K258011
direction: direction/reverse
start: 146
end: 1047
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2300232
display id: annotation2300232
version: 1
top level: BBa_K258011
location: https://synbiohub.org/public/igem/BBa_K258011/annotation2300232/range2300232/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258011/annotation2300232/range2300232/1
type: Range
persistent identity: range2300232
display id: range2300232
version: 1
top level: BBa_K258011
direction: direction/reverse
start: 1056
end: 1067
orientation: inline

BBa_K258010
type: SequenceAnnotation
persistent identity: annotation2300233
display id: annotation2300233
version: 1
top level: BBa_K258011
location: https://synbiohub.org/public/igem/BBa_K258011/annotation2300233/range2300233/1
component: BBa_K258010

https://synbiohub.org/public/igem/BBa_K258011/annotation2300233/range2300233/1
type: Range
persistent identity: range2300233
display id: range2300233
version: 1
top level: BBa_K258011
direction: direction/reverse
start: 1074
end: 1235
orientation: inline

BBa_K258006
type: SequenceAnnotation
persistent identity: annotation2300234
display id: annotation2300234
version: 1
top level: BBa_K258011
location: https://synbiohub.org/public/igem/BBa_K258011/annotation2300234/range2300234/1
component: BBa_K258006

https://synbiohub.org/public/igem/BBa_K258011/annotation2300234/range2300234/1
type: Range
persistent identity: range2300234
display id: range2300234
version: 1
top level: BBa_K258011
direction: direction/reverse
start: 1242
end: 2669
orientation: inline

BBa_I13500
type: SequenceAnnotation
persistent identity: annotation2300238
display id: annotation2300238
version: 1
top level: BBa_K258011
location: https://synbiohub.org/public/igem/BBa_K258011/annotation2300238/range2300238/1
component: BBa_I13500

https://synbiohub.org/public/igem/BBa_K258011/annotation2300238/range2300238/1
type: Range
persistent identity: range2300238
display id: range2300238
version: 1
top level: BBa_K258011
direction: direction/reverse
start: 2678
end: 3415
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2300245
display id: annotation2300245
version: 1
top level: BBa_K258011
location: https://synbiohub.org/public/igem/BBa_K258011/annotation2300245/range2300245/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K258011/annotation2300245/range2300245/1
type: Range
persistent identity: range2300245
display id: range2300245
version: 1
top level: BBa_K258011
direction: direction/reverse
start: 3424
end: 3552
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa Q04400
display id: BBa_Q04400
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0040.B0015.R0040)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: TetR QPI with strong RBS
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_C0040
component: BBa_R0040
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_Q04400_sequence/1

BBa_B0010
type: Component
persistent identity: component952797
display id: component952797
version: 1
top level: tetR
definition: BBa_B0010
access: public

BBa_C0040
type: Component
persistent identity: component952791
display id: component952791
version: 1
top level: tetR
definition: tetR
access: public

BBa_R0040
type: Component
persistent identity: component952822
display id: component952822
version: 1
top level: tetR
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component952807
display id: component952807
version: 1
top level: tetR
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component952781
display id: component952781
version: 1
top level: tetR
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952781
display id: annotation952781
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
type: Range
persistent identity: range952781
display id: range952781
version: 1
top level: tetR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation952791
display id: annotation952791
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
type: Range
persistent identity: range952791
display id: range952791
version: 1
top level: tetR
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952797
display id: annotation952797
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
type: Range
persistent identity: range952797
display id: range952797
version: 1
top level: tetR
direction: direction/forward
start: 712
end: 791
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952807
display id: annotation952807
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
type: Range
persistent identity: range952807
display id: range952807
version: 1
top level: tetR
direction: direction/forward
start: 800
end: 840
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation952822
display id: annotation952822
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
type: Range
persistent identity: range952822
display id: range952822
version: 1
top level: tetR
direction: direction/forward
start: 849
end: 902
orientation: inline

BBa_I13500
type: ComponentDefinition
persistent identity: BBa I13500
display id: BBa_I13500
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13500_sequence/1

BBa_B0034
type: Component
persistent identity: component1505000
display id: component1505000
version: 1
top level: BBa_I13500
definition: BBa_B0034
access: public

BBa_E0040
type: Component
persistent identity: component1505002
display id: component1505002
version: 1
top level: BBa_I13500
definition: GFP
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505000
display id: annotation1505000
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
type: Range
persistent identity: range1505000
display id: range1505000
version: 1
top level: BBa_I13500
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505002
display id: annotation1505002
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
type: Range
persistent identity: range1505002
display id: range1505002
version: 1
top level: BBa_I13500
direction: direction/forward
start: 19
end: 738
orientation: inline

BBa_K258006
type: ComponentDefinition
persistent identity: BBa K258006
display id: BBa_K258006
version: 1
was generated by: iGEM to SBOL conversion
description: Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1
created: 2009-10-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.
owned by: james
owned by: myers
mutable description: TliA has four glycinerich repeats (GGXGXD) in its C-terminus, which appear in many ABC transporter-secreted proteins.Export of fusion proteins with the whole TliA through the ABC transporter was evident on the basis of lipase enzymatic activity. Upon supplementation of E. coli with ABC transporter, EGF-TliA was excreted into the culture supernatant.Whole TliA were attached to C-termini of model proteins and enabled the export of the model proteins such as GFP and EGF which has 3 disulfide bonds in E. coli supplemented with ABC transporter. Activity domain (residues 1???268) and secretion/chaperon domain (residues 279???476). In our experiment, we observed that TliA fused proteins were excreted to supernatant culture succesfully by detecting lipase activity with tributyrin and spectrophotometric detection with the substrate p-nitrophenyl phosphate at 420 nm. At the experiments, Tlia fused proteins were excreted ten-fold more with ABC transporter PrtDEF of Erwinia chrysanthemi.
discontinued: false
dominant: false
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The sequence was optimized for E.coli with Biobrick restriction sites
star: false
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K258006_sequence/1

BBa_K258010
type: ComponentDefinition
persistent identity: BBa K258010
display id: BBa_K258010
version: 1
was generated by: iGEM to SBOL conversion
description: Human Epidermal growth factor (hEGF)
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: EGF is synthesized in human and some mammalians. Our EGF was synthesized synthetically at GENEART
owned by: james
owned by: myers
mutable description: Epidermal growth factor (EGF) is a single chain, 53 aminoacid-residue polypeptide (molecular weight 6045) and three intramolecular disulfide bonds. EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. EGF results in cellular proliferation, differentiation, and survival. EGF stimulates the growth of skin and corneal epithelium in vivo and in organ cultures. Epidermal growth factor (EGF) stimulates the growth of keratinocytes in vivo, and therefore plays an important role in the process of wound healing that depends on mitosis and migration of keratinocytes. Rhinewald and Green showed, in vitro that in the presence of growth factors , higher percentage of cells leave the resting state, enter and remain in the mitotic cycle. Mitogenic effect of EGF requires continuous exposure of target cells to EGF for a minimum 6-12 hours. The stimulation of wound healing by EGF has been confirmed as growth of granulation tissue in sponge implants used as inductive matrices. They observed, in rats, dose-dependent effect of EGF on granulation tissue formation as increased amounts of nucleic acids, and accumulation of collagen and glycosaminoglycans.
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: EGF with Biobrick standart restriction sites
star: false
creator: Cihan Tastan and Ozkan IS
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K258010_sequence/1

BBa_K258005
type: ComponentDefinition
persistent identity: BBa K258005
display id: BBa_K258005
version: 1
was generated by: iGEM to SBOL conversion
description: Oxygen promoter-Vitreoscilla hemoglobin(VHb) promoter in E. coli.
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: gene of Vitreoscilla hemoglobin(VHb). We synthesized synthetically at GENEART.
owned by: james
owned by: myers
mutable description: The promoter was maximally induced under microaerobic conditions (dissolved oxygen levels of less than 2% air saturation). Transcriptional activity decreased substantially under anaerobic conditions, suggesting the presence of a regulatory mechanism that is maximally induced under hypoxic but not completely anaerobic conditions in E.coli. Primer extension analysis was used to identify the existence of two overlapping promoterswithin a 150-base-pair region upstream of the structural VHb gene. The oxygen-dependent activity of both promoters was qualitatively similar, suggesting the existence of a common mechanism by which available oxygen concentrations influence expression from the two promoters. Oxygen-dependent control mechanisms revealed in some of the above studies include positive regulation by an activator protein. The bacterium Vitreo-scilla sp. is an obligate aerobe from the Beggiatoa family that synthesizes a hemoglobinlike molecule (VHb) in response to growth in oxygen-poor environments. The expression of the VHb gene (vgb) is regulated by oxygen in both its native host, Vitreoscilla, and in E.coli and is maximally induced under microaerophilic conditions (Dikshit and Webster 1988; Dikshit et al. 1992;Joshi and Dikshit 1994). The purposes were to characterize the response of the promoter to changes in oxygen availability in the environment and to obtain initial insights about the mechanism(s) by the promoter is controlled.
discontinued: false
dominant: false
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The palsmid id pMA (GENEART constantpalsmid with Amphicillin resistance and Biobrick restiction sites,EcoR1,Xba1,Spe1 and Pst1)
star: false
creator: Cihan Tastan
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K258005_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

https://synbiohub.org/public/igem/BBa_K258011_sequence/1
type: Sequence
persistent identity: BBa K258011 sequence
display id: BBa_K258011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagcttacaggacgctggggttaaagtatttgagttttgatgtggattaagtttagaggcaataaagattataataagtgctgctacaccatactgatgtatggcaaaccataataatgaacttaaggaagaccctctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacagcgactcggagtgtccactgtcacatgatggctattgcctgcacgatggcgtttgtatgtatatcgaggccctggacaaatatgcctgtaactgtgtggtgggctatatcggtgaacgttgccagtatcgtgacctgaaatggtgggaactgcgttactagatgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagctggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaagcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtttccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctctgggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggtgcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatctgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtgataaagtcctgaacatcggttacgaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04400_sequence/1
type: Sequence
persistent identity: BBa Q04400 sequence
display id: BBa_Q04400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13500_sequence/1
type: Sequence
persistent identity: BBa I13500 sequence
display id: BBa_I13500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258006_sequence/1
type: Sequence
persistent identity: BBa K258006 sequence
display id: BBa_K258006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagctggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaagcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtttccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctctgggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggtgcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatctgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtgataaagtcctgaacatcggttacgaaaacgacccggtctttcgtgctctggacggttccactttcaacctgtcctctctgggtgtacacgacaaagcccacgaatccactacggacaacattgtcagctttaacgaccactatgcgtctactctgtggaacgtcctgccgttctctattgccaacctgtccacttgggtgtctcacctgccgtctgcttacggtgacggcatgacccgtgttctggaaagcggcttctacgaacagatgacccgtgattccactattattgtggctaacctgtccgacccggcgcgcgctaatacgtgggttcaggatctgaaccgtaacgcagaaccgcataccggcaatactttcatcatcggttctgatggtaatgacctgattcagggcggtaaaggtgctgatttcatcgagggcggcaaaggtaatgacactatccgtgataattccggccacaatacgttcctgttttccggtcacttcggccaggaccgtattatcggctatcagccgaccgaccgtctggtgttccagggtgcggacggtagcaccgacctgcgtgaccacgcgaaagccgtgggcgctgacaccgttctgtctttcggcgcagactctgtgactctggtcggcgtcggcctgggtggcctgtggtctgaaggcgttctgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258010_sequence/1
type: Sequence
persistent identity: BBa K258010 sequence
display id: BBa_K258010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacagcgactcggagtgtccactgtcacatgatggctattgcctgcacgatggcgtttgtatgtatatcgaggccctggacaaatatgcctgtaactgtgtggtgggctatatcggtgaacgttgccagtatcgtgacctgaaatggtgggaactgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258005_sequence/1
type: Sequence
persistent identity: BBa K258005 sequence
display id: BBa_K258005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagcttacaggacgctggggttaaagtatttgagttttgatgtggattaagtttagaggcaataaagattataataagtgctgctacaccatactgatgtatggcaaaccataataatgaacttaaggaagaccctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z