LOCUS BBa_K258012 4760 bp DNA linear 01-Jun-2024 DEFINITION AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism ACCESSION BBa_K258012 VERSION BBa_K258012.1 FEATURES Location/Qualifiers misc_feature 250..254 /label=-24 region misc_feature 262..264 /label=-12 region promoter 1..275 /label=BBa_K131017 misc_feature 288..291 /label=conserved RBS 284..295 /label=BBa_B0034 CDS 305..1012 /label=cI lambda misc_feature 1013..1045 /label=LVA misc_feature 1052..1076 /label=Help:Barcodes CDS 302..1076 /label=BBa_C0051 stem_loop 1096..1139 /label=stem_loop misc_feature 1085..1164 /label=BBa_B0010 terminator 1085..1164 /label=BBa_B0010 stem_loop 1180..1199 /label=T7 TE misc_feature 1206..1206 /label=stop polyA_site 1200..1213 /label=polya misc_feature 1173..1213 /label=BBa_B0012 terminator 1173..1213 /label=BBa_B0012 terminator 1085..1213 /label=BBa_B0015 misc_feature 284..1213 /label=BBa_K082022 promoter 1259..1264 /label=-10 promoter 1236..1241 /label=-35 misc_feature 1246..1262 /label=OR1 misc_feature 1222..1238 /label=OR2 misc_feature 1222..1270 /label=BBa_R0051 promoter 1222..1270 /label=BBa_R0051 misc_feature 1283..1286 /label=conserved RBS 1279..1290 /label=BBa_B0034 misc_feature 1222..1290 /label=BBa_K235015 CDS 1297..1788 /label=BBa_K258004 misc_feature 1795..3222 /label=BBa_K258006 misc_feature 3235..3238 /label=conserved RBS 3231..3242 /label=BBa_B0034 misc_feature 3828..3859 /label=LVA CDS 3249..3827 /label=luxI misc_feature 3249..3866 /label=BBa_C0061 misc_feature 3867..3891 /label=Help:Barcodes CDS 3249..3866 /label=BBa_C0061 misc_feature 3231..3891 /label=BBa_C0261 misc_feature 3231..3891 /label=BBa_I751300 misc_feature 3904..3907 /label=conserved RBS 3900..3911 /label=BBa_B0034 CDS 3918..4592 /label=mrfp1 misc_feature 4599..4623 /label=Help:Barcodes CDS 3918..4623 /label=BBa_E1010 stem_loop 4643..4686 /label=stem_loop misc_feature 4632..4711 /label=BBa_B0010 terminator 4632..4711 /label=BBa_B0010 stem_loop 4727..4746 /label=T7 TE misc_feature 4753..4753 /label=stop polyA_site 4747..4760 /label=polya misc_feature 4720..4760 /label=BBa_B0012 terminator 4720..4760 /label=BBa_B0012 misc_feature 3900..4760 /label=BBa_K094130 ORIGIN 1 gtatcagcaa aaacactacg gtggataatc agtaaaacca tgaaactaga gccccgcaca 61 cttgcggggc tttttaattt tgaatttctt tcttattaaa acgccatttt tctgataaat 121 gtattagtag caatgcgcat ggtggcatat ttgcatcatt ttgcattttg caaatgcgat 181 ttgcaaaatg cgtgctcaat aaagcaccaa tatgcatcag gatcgaagaa aaaaggcgtt 241 tttaaaagtt ggcacgcatc gtgctttata cagattacta gagaaagagg agaaatacta 301 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 361 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 421 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 481 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 541 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 601 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 661 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 721 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 781 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 841 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 901 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 961 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa 1021 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact 1081 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1141 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1201 ttctgcgttt atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga 1261 taatggttgc tactagagaa agaggagaaa tactagatgt gtaacgacat gacccctgag 1321 caaatggcaa caaacgtgaa ctgtagtagt cctgaacgcc acactcgctc ttatgattat 1381 atggagggcg gggatattcg tgttcgtcgc ctgttctgcc gtactcaatg gtatctgcgc 1441 atcgataaac gtggtaaagt gaaaggcacc caagaaatga aaaacaacta taacatcatg 1501 gaaatccgta ccgtggcagt tggtattgta gcgatcaaag gcgtggaatc cgagttctat 1561 ctggcaatga acaaagaggg caaactgtat gccaaaaaag agtgtaacga ggactgtaac 1621 ttcaaagaac tgatcctgga gaaccattat aacacctatg ccagcgccaa atggacccat 1681 aacggtggtg aaatgttcgt ggccctgaac caaaaaggca ttccggtccg tggcaaaaaa 1741 acgaaaaaag agcagaaaac cgcccacttc ctgcctatgg ccattacata ctagatgggt 1801 gtgttcgatt acaagaacct gggtacggaa gcctctaaaa ccctgttcgc tgatgcgacc 1861 gcgatcaccc tgtataccta ccacaacctg gataacggtt tcgctgtagg ttatcagcag 1921 cacggcctgg gtctgggcct gccagcgacc ctggtcggcg cgctgctggg ttctaccgac 1981 tctcaaggcg tgatcccggg catcccatgg aacccggact ctgaaaaagc agctctggac 2041 gcggtacatg cagctggctg gactccgatc tccgcttctg cgctgggcta cggtggcaaa 2101 gtcgacgctc gtggcacctt cttcggcgaa aaagcaggct ataccacggc acaggccgaa 2161 gtactgggca agtacgacga tgctggtaaa ctgctggaaa tcggcatcgg tttccgtggc 2221 accagcggtc cgcgtgaatc cctgatcact gactccatcg gtgatctggt aagcgatctg 2281 ctggcggctc tgggtccgaa ggactacgcg aaaaactacg caggtgaagc cttcggtggt 2341 ctgctgaaga ctgttgcaga ctacgccggt gcccacggtc tgtccggtaa ggacgtcctg 2401 gtgtccggtc atagcctggg tggtctggcg gtgaactcta tggcagatct gagcaccagc 2461 aagtgggcgg gcttttacaa agatgcaaac tacctggctt acgcctctcc aacgcagtct 2521 gctggtgata aagtcctgaa catcggttac gaaaacgacc cggtctttcg tgctctggac 2581 ggttccactt tcaacctgtc ctctctgggt gtacacgaca aagcccacga atccactacg 2641 gacaacattg tcagctttaa cgaccactat gcgtctactc tgtggaacgt cctgccgttc 2701 tctattgcca acctgtccac ttgggtgtct cacctgccgt ctgcttacgg tgacggcatg 2761 acccgtgttc tggaaagcgg cttctacgaa cagatgaccc gtgattccac tattattgtg 2821 gctaacctgt ccgacccggc gcgcgctaat acgtgggttc aggatctgaa ccgtaacgca 2881 gaaccgcata ccggcaatac tttcatcatc ggttctgatg gtaatgacct gattcagggc 2941 ggtaaaggtg ctgatttcat cgagggcggc aaaggtaatg acactatccg tgataattcc 3001 ggccacaata cgttcctgtt ttccggtcac ttcggccagg accgtattat cggctatcag 3061 ccgaccgacc gtctggtgtt ccagggtgcg gacggtagca ccgacctgcg tgaccacgcg 3121 aaagccgtgg gcgctgacac cgttctgtct ttcggcgcag actctgtgac tctggtcggc 3181 gtcggcctgg gtggcctgtg gtctgaaggc gttctgattt cttactagag aaagaggaga 3241 aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca tcggaggagt 3301 ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag tgggacttag 3361 ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa tatatttatg 3421 cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca acaggtgatt 3481 atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc aaagatccta 3541 atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata aataactctg 3601 ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt agtcaaggta 3661 ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag cgtattaaag 3721 ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact aaatcggttg 3781 tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct gcaaacgacg 3841 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga 3901 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca 3961 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag 4021 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc 4081 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac 4141 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac 4201 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag 4261 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg 4321 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg 4381 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg 4441 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa 4501 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg 4561 aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat 4621 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 4681 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 4741 tgggcctttc tgcgtttata //